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Indirect measurement method of the small intestine power of the big rat

A small intestine and power technology, applied in the field of measuring the physiological information of small animals, can solve the problems of reduced accuracy, missed detection of activated carbon, and low accuracy, and achieve the effects of low cost, stable physical and chemical properties, and low price

Inactive Publication Date: 2008-02-06
楼征
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above method uses the naked eye to observe the ratio between the black-stained intestinal segment and the whole intestinal tract. The disadvantage of this method is that it will lead to missed detection of activated carbon that has entered the distal intestinal cavity but cannot be judged by naked eyes. In addition, the accuracy of visual inspection is relatively low. low, these factors will inevitably lead to a decrease in accuracy

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  • Indirect measurement method of the small intestine power of the big rat

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Embodiment 1

[0019] A method for indirect measurement of small intestinal motility in rats, comprising the following steps: adding 2000 blue dextran with a volume of 1 / 3-1 / 2 times of rat stomach capacity and a mass concentration of 1-4% to rat living bodies For intragastric administration, the volume dosage of blue dextran 2000 can be selected as 9 / 24 times, 5 / 12 times, 11 / 24 times of the rat stomach capacity, and the mass concentration can be selected as: 1.2%, 2%, 2.3% , 3%, blue dextran 2000 can adopt the DextranBlue-2000 (abbreviated BD-2000) produced by Sweden Pharmacia Company, wait for 1-1.5 hours, the time can be selected as 1 hour, 1.2 hours, 1.3 hours, 1.4 hours, put to death For rats, immediately take the entire small intestine from the pylorus to the terminal ileum of the above-mentioned rat, and divide the above-mentioned entire small intestine into at least 6 segments under tension-free conditions, such as 6 segments, 7 segments, 8 segments, 9 segments, and 10 segments, Secti...

Embodiment 2

[0021] A method for indirect measurement of small intestinal motility in rats, comprising the following steps: adding 2000 blue dextran with a volume of 1 / 3-1 / 2 times of rat stomach capacity and a mass concentration of 1-4% to rat living bodies For intragastric administration, the volume dosage of blue dextran 2000 can be selected as 9 / 24 times, 5 / 12 times, 11 / 24 times of the rat stomach capacity, and the mass concentration can be selected as: 1.2%, 2%, 2.3% , 3%, blue dextran 2000 can adopt the DextranBlue-2000 (abbreviated BD-2000) produced by Sweden Pharmacia Company, wait for 1-1.5 hours, the time can be selected as 1 hour, 1.2 hours, 1.3 hours, 1.4 hours, put to death For rats, during this period of time, blue dextran 2000 migrated through the peristalsis of the rat small intestine, thereby forming a certain concentration distribution in the small intestine, and then immediately took the entire small intestine from the pylorus to the terminal ileum of the above-mentioned r...

Embodiment 3

[0026] Healthy male SD rats were orally infused with 0.4ml of 2% BD-2000 solution, and killed immediately after 1 hour. Immediately after the death, the entire intestinal tube from the pylorus to the terminal ileum was taken out by laparotomy, and the total length of the small intestine was measured under tension-free conditions. The small intestine is equally divided into 10 equal parts, and each segment of the intestinal tube is cut along the mesenteric border, and the contents of the intestine are thoroughly washed with 4ml of double distilled water for each segment of the intestinal tube, and the flushing solution is collected into a centrifuge tube, and then rotated at 4000rpm. After centrifugation for 10 min, 1 ml of the supernatant after centrifugation was taken and put into a quartz cuvette, and the absorbance was detected under the condition of 620 nm. The corresponding relationship between the detected concentration and the corresponding position is shown in Figure 1,...

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Abstract

The invention discloses a method for indirect measurement of small intestine motility of rat, wherein blue glucan 2,000 with the volume being one third to one half of the stomach capacity of rat and mass concentration being one percent to four percent is filled into the stomach of rat living body; and then, the rate is kill after waiting 1 to 1.5 hours and small intestine from pylorus to the end of ileum of the rat are taken out instantly to be equally divided into at least six segments under the conditions of non-tension; then, contents inside each segment of small intestine are flushed out by double distilled water and the flushed fluid is collected to be centrifuged ten to fifteen minutes; finally, absorbance detection of the centrifuged supernatant is respectively completed to obtain distribution status of blue glucan 2,000 inside small intestine. The invention obtains concentration distribution curve reflecting small intestine motility of rat by use of spectrophotometer, thereby being helpful to analyze intestine motility function of rat more accurately; meanwhile, due to adopting blue glucan 2,000 as indicator, the invention is low in price and has no radioactivity, thereby doing no obvious harm to human body and being of high safety.

Description

technical field [0001] The invention relates to a method for measuring physiological information of small animals, in particular to a method for indirectly measuring small intestine dynamics of rats. Background technique [0002] The functional status of intestinal motility is one of the factors affecting the course and prognosis of critically ill patients. Once intestinal motility is restored, enteral nutrition can be given, which is of great significance for maintaining the integrity of the intestinal mucosal barrier. Therefore, an accurate assessment of the intestinal motility and functional status has important reference value for the study of intestinal functional status, thus indirectly providing clinical treatment basis. In the past, methods such as carbon ink and gavage of activated carbon were used to study the intestinal motility function of rats. The above method uses the naked eye to observe the ratio between the black-stained intestinal segment and the whole in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/22
Inventor 楼征
Owner 楼征