Novel plasmid autonomously replicable in enterobacteriaceae family

A technology of Enterobacteriaceae, Enterobacter genus, applied in the field of new plasmids
CN101128479BInactive Publication Date: 2011-05-11AJINOMOTO CO INC
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
AJINOMOTO CO INC
Publication Date
2011-05-11
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

A gene isolated from a microorganism selected from the group consisting of Pantoea bacteria, Erwinia bacteria, and Enterobacter bacteria, and encoding a Rep protein or a homologue thereof is described. A plasmid containing the gene which is autonomously replicable in Enterobacteriaceae bacteria is also described. A Enterobacteriaceae microorganism containing the plasmid is also described.
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Description

technical field

[0001] The present invention relates to novel plasmids, which are autonomously replicable in microorganisms from the family Enterobacteriaceae, and to methods of using the plasmids and microorganisms containing the plasmids. Background technique

[0002] Currently, the cultivation and improvement of microorganisms from the family Enterobacteriaceae, including Escherichia coli, by DNA recombinant technology is underway. In order to breed and improve microorganisms by DNA recombination technology, plasmids and broad host spectrum vectors derived from microorganisms of different genera are often used. Generally, however, plasmids from microorganisms with similar properties are used.

[0003] Plasmids derived from microorganisms of the family Enterobacteriaceae include small plasmids such as pBR322, RSF1010 (Gene, vol.75, (2) pp.271-288, 1989) and pUC (Gene, Oct. 1982, 1989) all derived from Escherichia coli. (3): 259-68), pHCM2 derived from Salmonella (Salmone...

Claims

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