Enterobacter strain capable of dissolving phosphorus and application of enterobacter strain

A technology of Enterobacter genus and strains, which is applied in the field of insoluble phosphate biodegradation, can solve the problems such as unsatisfactory effect of phosphorus-solubilizing bacteria, and achieve the effects of alleviating phosphorus crisis, good tolerance, and improving utilization efficiency

Active Publication Date: 2017-01-11
YUNNAN PHOSPHATE CHEM GROUP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent has a good effect on degrading phosphorus salts in soil, due to the large variety and high content of insoluble inorganic phosphates in the scrubbing tailings, the effect of the phosphorus-dissolving bacteria on the degradation of the scrubbing tailings is not ideal.

Method used

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  • Enterobacter strain capable of dissolving phosphorus and application of enterobacter strain
  • Enterobacter strain capable of dissolving phosphorus and application of enterobacter strain
  • Enterobacter strain capable of dissolving phosphorus and application of enterobacter strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The acquisition of embodiment 1 inorganic phosphobacteria strain YA-2

[0041] 1. The isolation and screening process of inorganic phosphate-solubilizing bacterial strain YA-2 is as follows:

[0042] (1) The soil was collected from the rhizosphere of weeds in the vegetation reclamation area of ​​Kunyang Phosphate Mine, Jinning County, Kunming City, Yunnan Province. The collected soil samples were put into a sealed bag by shaking the soil and immediately brought back to the laboratory for refrigeration for later use.

[0043] Inorganic phosphorus solid medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MnSO 4 .4H 2 O0.03g, FeSO 4 .7H 2 O 0.03g, MgSO 4 .7H 2 O 0.3g, Ca 3 (PO 4 ) 2 10g, 18g agar, 1000mL distilled water, pH7.0-7.5.

[0044] Inorganic phosphorus liquid medium: glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MnSO 4 .4H 2 O0.03g, FeSO 4 .7H 2 O 0.03g, MgSO 4 .7H 2 O 0.3g, Ca 3 (PO 4 ) 2 10g, distilled water 1000mL...

Embodiment 2

[0053] The optimization of embodiment 2 culture medium

[0054] (5) Shake flask fermentation: Pick a ring of strains from the preservation medium, put it in a sterilized 150mL triangular flask containing 50mL seed medium, and place it at 28°C, 180r.min -1 Incubate overnight.

[0055] (6) Screening of the best carbon source and nitrogen source: On the basis of the original inorganic phosphorus liquid medium, the carbon source (glucose, sucrose, maltose, soluble starch, the addition amount is 10g / L) and the nitrogen source (ammonium sulfate 0.05g / L, potassium nitrate 0.0644g / L, ammonium nitrate 0.0303g / L, urea 0.0277g / L) for screening, 1*10 5 Sterilize under Pa condition for 30min, inoculate the seed liquid in the culture medium according to the inoculation amount of 1.5%, place the constant temperature shaker (180r.min -1 , 28°C) for 7 days on a shaking table, and the molybdenum blue colorimetric method was used to measure the available phosphorus content in the solution. At...

Embodiment 3

[0059] Example 3 bacterial strain acid phosphatase activity and organic acid total amount under different phosphorus sources

[0060] Determination of Phosphatase Activity of Bacteria under Different Phosphorus Sources

[0061]The making of standard curve: take 6 test tubes, absorb lg / L standard p-nitrophenol solution successively. 0, 1, 2, 3, 4, and 5mL were placed in test tubes respectively, and the volume was adjusted to 5mL with sterile water. Add 1 mL of 0.5 mol / L calcium chloride and 4 mL of sodium hydroxide to each test tube, shake and filter, and measure the absorbance of the filtrate at 420 nm. The number of micromoles that act on the substrate and release the product in IL fermentation broth (culture broth) per hour is called 1 enzyme activity unit, denoted as umolL -1 h -1 , abbreviated as Eu.

[0062] Take 1mL of fermentation broth, add 4mL of MUB with a pH value of 6.5, and add 0.025mol.L -1 Disodium p-nitrophenyl phosphate solution 1mL, shake for a few secon...

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Abstract

The invention discloses an enterobacter strain capable of dissolving phosphorus and application of the enterobacter strain. A classification name of the enterobacter strain is enterobacter YA-2 (enterobacter sp.YA-2), the enterobacter strain is preserved in the China Center for Type Culture Collection on March 7, 2016, and a preservation number of the enterobacter strain is CCTCC NO:M 2016094. The enterobacter strain is applicable to scrubbing tailings and is also applicable to other insoluble phosphate and phosphorus-deficiency soil. The enterobacter strain and the application have the advantages that the insoluble phosphate in the tailings can be scrubbed and converted into soluble phosphate by the enterobacter strain, and accordingly the utilization efficiency of the scrubbed tailings can be improved; the optimally appropriate growth conditions, the saline-alkaline resistance, the temperature resistance and the like of the enterobacter strain are researched, the enterobacter strain is inoculated in acid red soil and is high in colonization ability, the dry weights and the fresh weights of Chinese cabbages are obviously increased, and accordingly the enterobacter strain is suitable to be applied to increasing the yield of crops.

Description

technical field [0001] The invention relates to the technical field of biodegradation of insoluble phosphate in phosphorus tailings, in particular to an enterobacter strain with phosphorus-dissolving ability and application thereof. Background technique [0002] Phosphorus is one of the essential nutrients for plants and an important component of compounds in plant cells. The total phosphorus content in plants accounts for 0.1-0.5% of the dry weight. There is no volatilization loss, and the leaching loss is also small. The excess phosphorus in the soil is basically fixed and becomes soil accumulation state P. Phosphate fertilizers are easily fixed in the soil and easily interact with Ca in the soil 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, the interest rate of P fertilizer is not high in the current season, generally only 10-20% or lower, but its accumulation and utilization rate is relatively high. [0003] Since 2010, the problems faced by the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G1/00C12R1/01
CPCC12N1/205C12R2001/01
Inventor 达布希拉图曾婕李云驹张晖宗世荣张可欣李若兰冯晓军
Owner YUNNAN PHOSPHATE CHEM GROUP CORP
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