Royal jelly enzymolysis product with moisture-absorbing moisture-keeping function
A technology of enzymatic hydrolysis product and moisturizing function, which is applied in the field of royal jelly enzymatic hydrolysis product, can solve the problems of worsening trade terms and low added value of products, and achieve the effect of increasing added value, good moisture absorption and moisturizing performance, and strengthening effect
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Embodiment 1
[0014] Weigh 25g of royal jelly, add water to dissolve, adjust the pH to 7, control the substrate concentration to 20%, add trypsin equivalent to 1.5% of the substrate concentration, control the enzymolysis temperature at 50°C, and the enzymolysis time for 7 hours. Keep the solution homogeneous. After the reaction, the system was placed in a water bath at 85° C. for 20 minutes to inactivate the enzyme, and the obtained product was filtered and freeze-dried.
[0015] Respectively take ribonuclease A (molecular weight 13700), insulin (molecular weight 5800), thymosin α1 (molecular weight 3108), somatostatin (molecular weight 1638) as molecular weight standard solutions, high performance liquid chromatography, the obtained Products with a relative molecular weight figure 1 .
Embodiment 2
[0017] Weigh 25g of royal jelly, add water to dissolve, adjust the pH to 8, control the substrate concentration to 8%, add trypsin equivalent to 0.5% of the substrate concentration, control the enzymolysis temperature to 40°C, and the enzymolysis time to 5 hours. Keep the solution homogeneous. After the reaction, the system was placed in a water bath at 85° C. for 20 minutes to inactivate the enzyme, and the obtained product was filtered and freeze-dried.
[0018] Respectively take ribonuclease A (molecular weight 13700), insulin (molecular weight 5800), thymosin α1 (molecular weight 3108), somatostatin (molecular weight 1638) as molecular weight standard solutions, high performance liquid chromatography, the obtained Products with a relative molecular weight figure 2 .
Embodiment 3
[0020] Weigh 25g of royal jelly, add water to dissolve, adjust the pH to 9, control the substrate concentration to 2%, add trypsin equivalent to 2.5% of the substrate concentration, control the enzymolysis temperature at 55°C, and the enzymolysis time for 2 hours. Keep the solution homogeneous. After the reaction, the system was placed in a water bath at 85° C. for 20 minutes to inactivate the enzyme, and the obtained product was filtered and freeze-dried.
[0021] Respectively take ribonuclease A (molecular weight 13700), insulin (molecular weight 5800), thymosin α1 (molecular weight 3108), somatostatin (molecular weight 1638) as molecular weight standard solutions, high performance liquid chromatography, the obtained Products with a relative molecular weight image 3 .
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