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Cultivated silkworm bmspz-like gene

A silkworm and gene technology, applied in the field of molecular biology, can solve the problem that there is no research report on the silkworm bmspz-like gene.

Inactive Publication Date: 2008-03-12
重庆拓桑生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the bmspz-like gene in silkworm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0045] Using the amino acid sequence encoded by the Drosophila Spatzle gene to homologously search the silkworm EST database and the gene prediction database (gene data obtained from the genome sequence prediction of the silkworm in our laboratory), a sequence with high homology was obtained, named bmb001, and then used this The sequence is the seed sequence. The silkworm EST library was searched using the BLASTn program, and the obtained homologous EST was electronically extended using DNASTAR. The homology retrieval and extension steps were repeated, and finally a contig with a length of 1150 bp was assembled. Using the silkworm cDNA that has pupated for one day as a template, carry out RT-PCR amplification with the aforementioned specific primers P1 and P2, and the PCR product is analyzed by 1% agarose gel electrophoresis, and a specific band of about 1000 bp appears. It is consistent with the electronic extension results. Connect with pMD18-T vector to transform Escherichi...

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PUM

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Abstract

The invention provides a bmspz-like gene of silkworms. A full-length cDNA sequence of silkworm Spaetzle (bmspz) gene is obtained based on existing network resource and by taking use of silkworm gene data and using a biologic information method (including homology inspection, specie-gathering and sequence splicing, etc.) to expand EST sequence. Such study as clone expression of bmspz is hopeful to be applied in anti-microbe infection of silkworms, is of high importance for preventing and treating diseases of silkworms and other insects, can also provide facility for studying the interaction between Spatzle and other signal molecules in the transmitting path of congenital immunity signals.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a key gene spz from the silkworm innate immune signal transmission pathway. Background technique [0002] Drosophila Toll-like protein was discovered in 1984, and the homologous human Toll-like receptor (TLR) protein (hTLR4) was discovered in 1997. The Toll receptor family recognizes different antigens respectively. The difference is that Drosophila Spatzle protein, as an essential ligand of Toll, forms a complex with Toll to cause the expression of Toll-dependent immune gene drosomycin, and activate Toll-dependent immune pathways. The Toll family is required for the antifungal immune response of Drosophila larvae and adults, and is an essential gene for the innate immune response. However, the specific signaling mechanism of its immune system remains unclear. At the same time, the Toll gene family plays a vital role in the embryonic development process, shouldering the importa...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/435
Inventor 夏庆友程廷才徐汉福向仲怀
Owner 重庆拓桑生物科技有限公司