Recombination expression carrier and method for soluble expressing human I-type metallothionin

A metallothionein and expression vector technology, applied to the recombinant expression vector expressing human type I metallothionein, in the field of expressing and producing human type I metallothionein in a soluble form, can solve the problem of expensive protease cost, time-consuming, inability to obtain MT production and other issues
CN101144093BActive Publication Date: 2011-06-15GUANGZHOU BIOPHARMACEUTICAL R&D CENT OF JINAN UNIV CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU BIOPHARMACEUTICAL R&D CENT OF JINAN UNIV CO LTD
Publication Date
2011-06-15

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Abstract

The present invention relates to a method for producing a human I type metal sulfur protein (Human metallothionein-I, hMT-I), in particular to the fusion expression by adopting the small molecule ubinquitin-related modifier mature peptide (Small Ubiquitin-related Modifier, SUMO) and hMT-I, and the fusion protein and the ubinquitin-related modifier protease 1 (Ubiquitin-like protease 1) are co-expressed in a prokaryotic organism. In the fermentation and expression process, the ubiquitin associative modifier gene protease 1 can hydrolyze the fusion protein which consists of the ubinquitin-related modifier mature peptide and the hMT-I, to produce the dissoluble hMT-I.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering. More specifically, the present invention relates to a recombinant expression vector for expressing human type I metallothionein and a method for expressing and producing human type I metallothionein in a soluble form. Background technique

[0002] Metallothionein (MT) is a class of low molecular weight, cysteine-rich, metal-binding proteins that can bind heavy metals and be induced by metals. Since metallothionein was first isolated in 1957, researchers have found that MT widely exists in the tissues of animals, plants and eukaryotic microorganisms.

[0003] There are 4 MT subtypes in mammals, namely MT-I, MT-II, MT-III, MT-IV, where MT-I and MT-II are present in all organs and MT-III is only present in brain tissue , while MT-IV is only present in squamous epithelial tissues (Uchida et al., J Biol. Chem., 1995, 270: 3365-3369). Typical mammalian MT has (1) low molecular weight, generally on...

Claims

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