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Reagent kit for detecting hyperlipemia susceptibility

A technology for hyperlipidemia and kits, which is applied in measurement devices, microbial determination/inspection, instruments, etc., can solve problems such as obstruction of lipid scavenging ability and decreased receptor binding ability.

Inactive Publication Date: 2008-03-19
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of association analyzes at home and abroad (more than 1000 Chinese populations) have shown that the genotype of the SNP site rs17247263 (position 156 in the sequence shown in SEQ ID NO: 2) on the APOB gene has a positive effect on both apolipoprotein and blood lipids. Influence, the rs17247263 site is a C / T polymorphism on the APOB gene, the low-frequency allele type T leads to a decrease in the ability of the encoded protein to bind to the LDLR receptor, and the ability to clear lipids in the plasma is hindered, and the low density The accumulation of lipoproteins can further cause atherosclerosis and coronary heart disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Use of detection kits

[0032] Step 1: Extraction of DNA template

[0033] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0034] Step 2: Real-time quantitative PCR reaction

[0035] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to hyperlipidemia, which contains the following primer pairs and fluorescent probes:

[0036] Sense primer 1: 5'-AACAGCCAGGTATAGGGAT-3' (SEQ ID NO: 4) with a Tm value of 56°C

[0037] Antisense primer 1: 5'-TATACACCAACCTCCTAATC-3' (SEQ ID NO: 5) with a Tm value of 56°C

[0038] Sense primer 2: 5'-TCAATTGGTTACAGGAGGC-3' (SEQ ID NO: 6) with a Tm value of 56°C

[0039] Antisense primer 2: 5'-GTATCGTTGAAGTTCCTGC-3' (SEQ ID NO: 7) with a Tm value of 56°C

[0040] Sense primer 3: 5'-AGGGTGAGATTCCAAGATAA-3' (SEQ ID NO: 8) with a Tm value of 56°C

[0041] Antisense primer 3: 5'-CAAGCAAATGACTAAAGAGAA-3' (SEQ ID NO: 9) with a Tm value of 56°C

[0042] Fluorescent probe 1: 5...

Embodiment 2

[0050] Example 2. Instructing people to actively prevent hyperlipidemia services

[0051] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0052] Step 1: DNA Extraction

[0053] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0054] Step 2: Genotyping Assays

[0055] Using the kit provided by the present invention, the rs17237904 SNP site on the CETP gene, the rs17247263 SNP site on the APOB gene and the rs320 SNP site on the LPL gene are simultaneously detected by fluorescence quantitative PCR on the CETP gene of the DNA of the subject to be tested. Genotypes of the three SNPs loci.

[0056] Step 3: Instruct people to proactively prevent hyperlipi...

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Abstract

The invention discloses a reagent kit to detect the susceptibility of the hyperlipoidemia. The reagent kit comprises a specific primer pair and a specific fluorescent probe which are used to detect a 146th positioned SNP locus in the CETP gene SEQ ID NO: 1, a 156th positioned SNP locus in the APOB gene SEQ ID NO: 2 and a 118th SNP site in the LPL gene SEQ ID NO: 3 synchronouslyand a routine component which is used for detecting fluorescent quantification PCR, etc. The reagent kit predicts the susceptibility of the individual of the hyperlipoidemia through detecting and analyzing the carrying type on the SNPs locus gene of the CETP gene, the APOB gene and the LPL gene of the individual synchronously.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to hyperlipidemia. The single nucleotide polymorphism (SNP) of lipoprotein B gene (apolipoprotein B, APOB) and lipoprotein lipase gene (lipoprotein lipase, LPL) was used to predict the susceptibility of individuals to hyperlipidemia. Background technique [0002] Due to abnormal fat metabolism or operation, one or several lipids in plasma are higher than normal, which is called hyperlipidemia (hyperlipidemia), which can be manifested as hypercholesterolemia (hypercholesterolemia), hypertriglyceridemia (hypertriglyceridemia) or Both (mixed hyperlipidemia). Lipids are insoluble or slightly soluble in water, and must be combined with proteins to exist in the form of lipoproteins in order to operate in the blood circulation. Therefore, hyperlipidemia is often a reflection of hyperlipoproteinemia (hyperlipoproteinemia). Since it is gr...

Claims

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Application Information

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IPC IPC(8): G01N21/00C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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