Method for extracting high pure chicoric acid from Coneflower

A kind of Echinacea purpurea, high-purity technology, applied in the direction of pharmaceutical formulations, separation/purification of carboxylic acid esters, medical preparations containing active ingredients, etc., can solve problems such as the inability to realize large-scale industrial production, and achieve high purity and high yield High, simple and fast operation

Inactive Publication Date: 2008-03-26
LIAOCHENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Large-scale industrial production is not yet possible

Method used

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  • Method for extracting high pure chicoric acid from Coneflower

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Ethanol extraction: Take 100 g of crushed dry Echinacea purpurea root, add 3-15 times the amount of 95% ethanol solvent, soak at room temperature for 24 hours, heat and reflux for 3 hours, and separate the medicinal liquid. Add 95% ethanol with 10 times the amount of medicinal dregs, heat and reflux for 2 hours, and separate the medicinal liquid. The extracted medicinal liquids are combined, and the impurities in the extraction liquid are removed by means of filtration and centrifugation. The extraction solvent is recovered by distillation or vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0022] Macroporous adsorption resin column pre-separation: put the ethanol extract obtained above on an AB-8 macroporous adsorption resin column, first wash with water until the effluent is colorless, then elute with 5% ethanol 10 times the column volume, collect 70% ethanol eluate, the solvent was recovered by distillation under reduced pressure, and th...

Embodiment 2

[0025] Take 100 g of crushed Echinacea purpurea roots, add 1.5 L of 40% ethanol, soak at room temperature for 4 hours, heat and reflux for 3 hours, and separate the medicinal liquid. Add 1 L of 95% ethanol, heat to reflux for 2 hours, and separate the liquid medicine. The extracted medicinal liquids are combined, and the impurities in the extraction liquid are removed by filtering. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0026] Put the ethanol extract obtained above on the AB-8 macroporous adsorption resin column, first wash with water until the effluent is colorless, then elute with 30% ethanol of 10 times the column volume, collect the 30% ethanol eluate, reduce The solvent is recovered by pressure distillation to obtain the cichoric acid extract.

[0027] Use petroleum ether-ethyl acetate-methanol-acetic acid water as the high-speed countercurrent chromatography two-phase solvent system to separate ...

Embodiment 3

[0033] Take 100 g of crushed Echinacea purpurea roots, add 1.5 L of 40% ethanol, soak at room temperature for 4 hours, heat and reflux for 3 hours, and separate the medicinal liquid. Add 1 L of 95% ethanol, heat to reflux for 2 hours, and separate the liquid medicine. The extracted medicinal liquids are combined, and the impurities in the extraction liquid are removed by filtering. The extraction solvent is recovered by vacuum distillation to obtain the ethanol extract of Echinacea purpurea.

[0034] Put the ethanol extract obtained above on the AB-8 macroporous adsorption resin column, first wash with water until the effluent is colorless, then elute with 30% ethanol of 10 times the column volume, collect the 30% ethanol eluate, reduce The solvent is recovered by pressure distillation to obtain the cichoric acid extract. The purity of the prepared cichoric acid can reach more than 99%, and the yield can reach more than 90%.

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Abstract

The present invention relates to process of extracting high purity chicoric acid from Echinacea purpurea. The process includes the following steps: 1. extracting crushed Echinacea purpurea root with alcohol and separating to eliminate solid impurity; 2. pre-separating the extracted liquid after recovering alcohol in a macroporous resin column through eluting with alcohol solution of different concentrations to obtain the extract; and 3. purifying the extract through one high speed counter current chromatographic process to obtain chicoric acid of purity over 99 %.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine preparation and a preparation method thereof, in particular to a method for preparing high-purity cichoric acid from Echinacea purpurea. 2. Background technology [0002] Echinacea purpurea is an immunostimulant and immunomodulator that has received widespread attention in the world, and its main physiologically active component is cichoric acid. Modern pharmacological studies have shown that cichoric acid has the effect of enhancing immune function and anti-inflammation, can inhibit hyaluronidase, protect collagen III from free radicals that can cause degradation, and has the effect of inhibiting HIV-1 and HIV-1 integrase . [0003] The method of extracting and separating cichoric acid from Echinacea purpurea has been reported in the literature, such as Wu Qilin et al. [Research on the preparation of cichoric acid by applying macroporous adsorption resin adsorption separation technology; Jour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48A61K36/28
Inventor 孙爱玲柳仁民
Owner LIAOCHENG UNIV
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