Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of magnolia bark and its active ingredient in preparing medicament for treating tumour stem cell

A technology of tumor stem cells and effective components, which is applied to the application field of Magnolia officinalis and its effective components in the preparation of medicines for treating tumor stem cells, can solve the problems such as not being discovered, and achieve the effect of good curative effect and great development prospect.

Inactive Publication Date: 2008-04-02
HANGZHOU HEBO BIOTECH
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the application of magnolia bark in the preparation of drugs for the treatment of tumor stem cells has not been discovered

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of magnolia bark and its active ingredient in preparing medicament for treating tumour stem cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Preparation of Magnolia officinalis ethanol extract

[0017] Percolation method: take 5000g magnolia officinalis dried skin powder and percolate with 6 times the amount of 95% ethanol, and the percolation speed is 15ml / min. Recover the ethanol leachate, recover the ethanol under reduced pressure until there is no ethanol smell, concentrate to obtain the extract, and set aside.

[0018] Generally, the ethanol concentration used here can be any value between 75% and 95%. The ethanol used in this embodiment can also be replaced by various alcohols such as methanol and propanol; other organic solvents such as ethyl acetate and ether solvents can also be used. replace

Embodiment 2

[0019] Embodiment 2: Magnolia officinalis supercritical extract preparation supercritical extraction: Magnolia officinalis 200g is pulverized into coarse powder (crossing 40 mesh sieves), puts 1L supercritical CO 2 In the extraction kettle, adjust the pressure: 25MPa, temperature: 45°C, flow rate: 30kg / h, extraction time: 2h. Collect the extracts.

Embodiment 3

[0020] Example 3: Determination of the efficacy of Magnolia officinalis on tumor stem cells

[0021] Experimental method: peripheral blood or bone marrow cells of patients with acute myeloid leukemia and blast criss chronic myeloid leukemia. Peripheral blood cells use leukocyte separation medium to remove red blood cells, and bone marrow cells use 150mM NH 4 Cl and 10 mM NaHCO 3 Red blood cells were removed by treatment for 5 minutes. The cells were washed three times with serum-free medium, and the ethanol extract of Magnolia officinalis obtained in Example 1 (final concentration was 0, 5, 10 μg / ml) was added to treat for 4 hours, or cytarabine was added (final concentration was 0, 5, 10 μg / ml). Then wash three times with serum-free medium to remove Magnolia officinalis ethanol extract, and use methylcellulose IMDM culture fluid (1% methylcellulose, 30% bovine serum albumin, 0.2mM mercaptoethanol, 2mM glutamine , 50ng / ml recombinant human stem cell factor, 10 / ml recombina...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides the application of an officinal magnolia and the effective components of the officinal magnolia in preparing the stem cell for remedying the tumour. The application of the officinal magnolia and the effective components of the officinal magnolia of the invention in preparing the stem cell for remedying the tumour has the main beneficial effects of prompting the new purpose of the officinal magnolia and the effective components of the officinal magnolia, providing new drug filtration with the base and the good curative effect, and the invention has significant developing prospect.

Description

(1) Technical field [0001] The invention relates to the application of magnolia bark and its effective pharmacological components in the preparation of medicines for treating tumor stem cells. (2) Background technology [0002] Tumor cells in tumors can be divided into two categories. One is normal tumor cells and the other is cancer stem cells. Ordinary tumor cells have the characteristics of rapid division, sensitivity to anticancer drugs, and no self-renewal ability. Therefore, normal tumor cells die after dividing for a certain number of generations. Cancer stem cells have the following characteristics: they are usually in a quiescent state, that is, they do not divide, they are not sensitive to anticancer drugs, and they have the ability to renew themselves, that is, they have the ability to reproduce indefinitely. In cancer chemotherapy, a large number of tumor cells are killed (because they are sensitive to chemotherapy drugs), but tumor stem cells will survive (be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/575A61K31/05A61P35/00
Inventor 胡汛
Owner HANGZHOU HEBO BIOTECH