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Molecule mark for assisting root-knot nematodes N resistant gene selection

A molecular marker and assisted selection technology, applied in the direction of plant genetic improvement, application, recombinant DNA technology, etc., can solve the problems of being easily affected by the environment and low selection efficiency.

Active Publication Date: 2008-04-02
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding methods are generally susceptible to environmental influences and low selection efficiency

Method used

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  • Molecule mark for assisting root-knot nematodes N resistant gene selection
  • Molecule mark for assisting root-knot nematodes N resistant gene selection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1B

[0043] Embodiment 1BSA (Bulked Segregated Analysis, separation group analysis method):

[0044] The female parent of the analyzed population is the inbred line Carolina Wonder with the "N" gene; the male parent is the susceptible inbred line Solanum; the F1 generation is obtained by crossing the two, and then the F2 generation is obtained by selfing.

[0045] According to the results of disease resistance identification, the F 2 In the population, 10 extremely resistant and susceptible plants with the highest and lowest disease grade index were selected, and 100ng DNA was mixed from each plant to establish a resistant pool and a susceptible pool. Using the DNA of the resistant pool and the sensitive pool as a template, amplify according to the AFLP method to find the differential fragments of the two pools, and then perform AFLP amplification using the individual DNA of the F2 population as a template to explore the distribution of the differential fragments in the F2 populati...

Embodiment 2

[0118] Application of Embodiment 2 Mark E39 / M41-527

[0119]Using the above AFLP analysis method, the 282 individual plants with resistance and sensitivity were artificially inoculated and identified, and DNA marker analysis was performed at the same time, and repeated three times, the results were stable. The number of plants containing the marker was 230, the number of plants without the marker was 52, and the number of plants that did not match the results of disease resistance was 15, with an accuracy rate of 94.6.

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Abstract

The invention provides a molecular mark assisting selection of a root-knot nematode resistant N gene. The invention adopts a F2 population which containing the 'N' gene and is composed of inbred line Carolina Wonder and susceptible inbred line solanaceous phylum and artificial inoculated root-knob nematode, together with the BSA and AFLP technologies, to develop molecular marks and obtain a molecular mark E39 / M41-527.The primers augmenting the E39 / M41-527 are represented in the sequence table SEQ ID NO.1, and NO.2. According the analysis of AFLP, the molecular mark E39 / M41-527 shows polymorphism in a resistant-susceptible pool; and by the analysis of a Joinmap3.0 mapping software, the genetic distance of the N gene is 6.258 cm. The invention provides a foundation for selection of root-knot nematode resistant N gene in peppers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker that can be used to assist in the selection of the root-knot nematode N gene. Background technique [0002] my country is the country with the largest pepper planting area in the world. In 2002, the planting area reached 1.3 million hectares, and the output was about 28.19 million tons. Root-knot nematodes seriously damage peppers. Root-knot nematode mainly harms pepper production in southern my country, but in recent years, with the increase of production area in northern protected areas, the increase of multiple cropping index, and the widespread phenomenon of continuous cropping and heavy cropping, nematode diseases have spread rapidly to northern regions. More than 90% of crop losses caused by root-knot nematodes are caused by southern root-knot nematodes (M. .hapla) caused by the four most common species, and root-knot nematode incognita is the m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12P19/34A01H1/00
Inventor 张宝玺王立浩毛胜利顾晓慧
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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