Method for producing modified electrode by electro-deposition chitosan-ionic liquid-enzyme compound film

A technology for ionic liquids and modified electrodes, which is applied in the field of electrodeposited chitosan-ionic liquid-enzyme composite membranes to prepare modified electrodes, can solve the problems of long preparation period and complex preparation process of enzyme-modified electrodes, and achieve the promotion of electron transfer and easy Electrochemical and electrocatalytic reactions, thickness controllable effect

Inactive Publication Date: 2008-06-11
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

[0006] However, the preparation process of enzyme-modified electrodes...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Chitosan is dissolved in the acetic acid solution that mass percentage concentration is 1%, and the mass percentage concentration of chitosan is 0.5%, adds ionic liquid 1-butyl 3-methylimidazole boron tetrafluoride, and ionic liquid mass is shell 1% of the mass of the polysaccharide solution, magnetically stirred for 3 hours, ultrasonically dispersed for 30 minutes, then 1 mg / mL horseradish peroxidase was added, and ultrasonically dispersed for 15 minutes. The gold electrode is used as a cathode, and electrodeposited at a potential of -1V for 5 minutes to obtain an electrodeposited chitosan-ionic liquid-horseradish peroxidase composite film modified electrode, and the obtained modified electrode can be used in a biosensor.

Embodiment 2

[0025] Chitosan is dissolved in the hydrochloric acid solution that mass percent concentration is 1%, and the mass percent concentration of chitosan is 1%, and ionic liquid 1-butyl 3-methylimidazole boron tetrafluoride is added, and ionic liquid mass is shell 2% of the mass of the polysaccharide solution, magnetically stirred for 1 hour, ultrasonically dispersed for 10 minutes, then 2 mg / mL glucose oxidase was added, and ultrasonically dispersed for 10 minutes. The glassy carbon electrode is used as a cathode, and electrodeposited at a potential of -1.2V for 8 minutes to obtain an electrodeposited chitosan-ionic liquid-glucose oxidase composite membrane modified electrode, and the obtained modified electrode can be used in a biosensor.

Embodiment 3

[0027] Chitosan is dissolved in the acetic acid solution that mass percent concentration is 0.5%, and the mass percent concentration of chitosan is 1%, and ionic liquid 1-butyl 3-methylimidazolium chloride is added, and the mass percent concentration of ionic liquid is chitosan solution 1% by mass, magnetically stirred for 3 hours, ultrasonically dispersed for 30 minutes, then added with 1 mg / mL glucose oxidase and 2 mg / mL horseradish peroxidase, ultrasonically dispersed for 10 minutes. The platinum electrode is used as a cathode, and electrodeposited at a potential of -1.5V for 5 minutes to obtain an electrodeposited chitosan-ionic liquid-dual enzyme composite membrane modified electrode, and the obtained modified electrode can be used in a biosensor.

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PUM

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Abstract

The invention discloses an ectrodeposit chitosan-ionic liquid-enzyme complex film manufacturing and modifying electrode method, which is characterized in that: when manufacturing and modifying, the electrode is inserted into the acid solution containing chitosan, ionic liquid and enzyme, the ectrodeposit is conducted by energizing, the chitosan, ionic liquid and enzyme are adhered on the surface of electrode negative to obtain modified electrode. The method in the invention has mild condition and is simple and convenient, the thickness of chitosan-ionic liquid-enzyme complex film is controllable, and the enzyme fixing volume can be controlled according to needs.

Description

technical field [0001] The invention relates to the preparation of a modified electrode, in particular to a method for preparing a modified electrode by electrodepositing a chitosan-ionic liquid-enzyme composite film. Background technique [0002] Biosensor is a new type of sensor technology developed in recent decades. It has the advantages of fast analysis speed and convenient use. It has important application value in biomedicine, clinical diagnosis, industrial control, environmental monitoring, food and pharmaceutical industry and other fields. Enzyme-modified electrodes are a hotspot in biosensor research, and a variety of electrode preparation methods have been developed. The sensitivity and stability of enzyme-modified electrodes are two important indicators for evaluating the performance of enzyme-modified electrodes, and the controllability of the preparation process of enzyme-modified electrodes is the main factor that determines the practical application of enzyme...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 奚凤娜林贤福
Owner ZHEJIANG UNIV
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