Method for producing insect parasitic bacteria fruit body

A production method and technology of parasitic bacteria, applied in the direction of fungi, etc., can solve problems such as difficulty in obtaining ascospores, difficulty in producing ascospores, and difficulty in stable production, so as to improve the utilization value, decrease the inhibitory activity, and increase the infection rate Effect

Inactive Publication Date: 2008-07-02
近藤 幸盛
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Problems solved by technology

[0007] In addition, in the methods described in Japanese Patent Laid-Open No. 10-42691 and International Publication No. WO01/022801 pamphlet, it is also difficult to generate ascospores, and it is also difficult to ensure a stable ascospore as an inoculum source.
In other words, these methods have the following problems: insect parasites such as Cordyceps sinensis fungi produce less fruiting bodies, and in dry years, they are hardly seen even in the wild, and it is difficult to ensure stable product

Method used

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  • Method for producing insect parasitic bacteria fruit body
  • Method for producing insect parasitic bacteria fruit body
  • Method for producing insect parasitic bacteria fruit body

Examples

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reference example 1

[0057] Reference Example 1, Comparison of Insect Generations

[0058] Place the insect parasites in PDA medium, propagate at 25°C for 10 days, and spread them. The 3rd (age), 4th, and 5th instars, 10 prepupae and 10 pupae of Spodoptera litura larvae were placed in the Let it stand there for 2 days and inoculate Cordyceps militaris. 35 days after inoculation, feeding and management were performed to investigate whether Cordyceps militaris was infected, the number of dead insects, and whether fruiting bodies were produced. A test was also carried out in the case where the above treatment was not performed (no treatment). The results are listed in Table 1.

[0059] Table 1

[0060] generations

[0061] From the results shown in Table 1, it can be seen that 9 of the prepupa were infected, 2 of the 3rd instar larvae, and 1 of the 4th instar larvae were infected. Although it can be determined that there are infected in the prepupa and some larvae like this, in other ge...

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Abstract

The invention provides a method for rapidly and stably producing fruiting body of insect parasite. The method comprises the following steps of: injuring epidermis of an insect, inoculating an insect parasite at the injured section, and culturing the insect parasite in the insect body to obtain the fruiting body of the insect parasite. The insect parasite can be selected from, such as, Cordyceps sinensis, and the insect is selected from Lepidopteran insect such as Spodoptera litura. Preferably, the insect parasite is cultured in an agar culture medium or a grain culture medium to generate mycelia of the insect parasite and is inoculated by disposing the insect with injured epidermis on the insect parasite. Additionally, the insect parasite can propagate in the insect body, so that the insect is hardened and the fruiting body is generated using the portion of the epidermis where a fruiting body primodium is generated as the seed.

Description

technical field [0001] The present invention relates to a method for rapidly and stably producing fruiting bodies (mushrooms) of insect parasitic fungi such as Cordyceps sinensis fungi, which are utilized as health food, pharmaceuticals, cosmetics, etc. Background technique [0002] Fruiting bodies of insect parasitic fungi, such as Cordyceps sinensis, can be used as traditional Chinese medicine and high-grade food materials. The fruiting bodies of Cordyceps sinensis fungi are usually picked and grown naturally, so the number is often small, the size is different, and the quality is not stable. In addition, even if the insect parasites are of the same genus as Cordyceps militaris, they often have differences in growth rate, fungal growth and entomopathogenicity. Therefore, people have been exploring excellent insect parasites and studying their artificial culture methods. [0003] Specifically, it has been proposed that host insects are artificially inoculated with Cordyce...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 近藤幸盛田口义广
Owner 近藤 幸盛
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