Method for improving electrophoresis resolution capacity and sequencing quality based on nano particle
A nanoparticle and resolution technology, which is applied in the direction of measuring devices, material analysis through electromagnetic means, material analysis, etc., can solve the problems that cannot be adjusted and changed, and the preparation process is complicated, so as to achieve simple use, simple method, and lower sequencing price Effect
Inactive Publication Date: 2011-01-05
SHANGHAI JIAO TONG UNIV
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Problems solved by technology
Its shortcoming is: it is necessary to introduce colloidal gold particles into the polymer used for DNA separation through complex chemical methods to prepare composite media, and it must be packed in capillary in advance.
Not only the preparation process is complicated, but also it cannot be adjusted and changed according to the purpose of use during use
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Abstract
The invention relates to a method for improving the electrophoresis distinguishability and sequence detecting quality based on nanometer grains in the technique field of biological engineering. By adding colloid gold into a sample or an sample buffer solution during the sample process, the invention leads colloid gold particles to be combined with single-chain DNA molecules for one aspect to enrich DNA molecules and increase the moving speed of DNA and leads the colloid gold particles in a polymer network of a capillary to form an interlinkage point for the other aspect, thereby enhancing thevalidity of separation. The particle diameter of the colloid gold is between 5nm to 60nm and the dosage of the colloid gold is between 0.001nmol / l to 1nmol / l. The method has the advantages of remarkable effect of optimizing electrophoresis, simple preparation and broad application, etc.
Description
A method for improving electrophoretic resolution and sequencing quality based on nanoparticles technical field The invention relates to a sample preparation method for electrophoresis in the technical field of bioengineering, in particular to a method for improving electrophoresis resolution and sequencing quality based on nanoparticles. Background technique Electrophoresis technology is to use the electric field to separate, identify or purify the sample due to the difference in the charging properties of various molecules in the sample to be separated and the differences in the size and shape of the molecule itself, so that the charged molecules have different migration speeds. technology. It has been widely used in analytical chemistry, biochemistry, clinical chemistry, toxicology, pharmacology, immunology, microbiology, food chemistry and other fields. The resolution of electrophoresis is one of the most important indicators. Capillary electrophoresis technology, a...
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IPC IPC(8): G01N27/447C12Q1/68
Inventor 李雪玲吕军鸿胡钧
Owner SHANGHAI JIAO TONG UNIV