Use of a composition which regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process

A composition and cell technology, applied in the field of sorting and staining sperm cells, can solve the problems of reduced forward motility and the like
CN101238369BActive Publication Date: 2012-07-04INGURAN LLC
24 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INGURAN LLC
Publication Date
2012-07-04

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention provides staining mixtures comprising viable spermatozoa, a composition which regulates oxidation / reduction reactions intracellularly or extracellularly, and a DNA selective dye are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enrichedpopulations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for staining sperm cells comprising the formation of a staining mixture are alsodisclosed.
Need to check novelty before this filing date? Find Prior Art

Description

field of invention

[0001] The present invention generally relates to methods of sorting stained spermatids. More specifically, the present invention relates to a method of sorting sperm cells, wherein a sperm cell suspension is formed containing a composition that modulates intracellular and / or extracellular oxidation / reduction reactions. Herein, sperm refers to non-human sperm. Background of the invention

[0002] Fertilization of animals by artificial insemination (AI) and embryo transfer after in vitro fertilization is a well-established technology. In the livestock industry, there are clear advantages in being able to influence reproductive outcome so that offspring possess one or more desired characteristics. For example, in the dairy industry, if the offspring can be pre-selected in favor of females and the production of cows can be guaranteed, it will bring economic benefits. Separating sperm into enriched cell populations with X and Y chromosomes, the so-called se...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More