Medicine carrying fibroin microsphere and preparation thereof

A technology of drug-loaded microspheres and silk fibroin, applied in the fields of biomedicine and polymer chemistry, can solve the problems of reproduction and the integrity of biological macromolecules, such as side effects, and achieve stable performance

Inactive Publication Date: 2008-08-20
SUZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In the Chinese invention patent with the publication number CN1293952A, chitosan and fibroin blended microspheres and their use and preparation method are disclosed, using a composite carrier and a cross-linking agent glutaraldehyde, utilizing glutaraldehyde, chitosan and silk Drug-encapsulated microspheres were prepared by cross-linking cardiotin, and the cross-linking effect of glutaraldehyde has side effects on cell proliferation and the integrity of biomacromolecules

Method used

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  • Medicine carrying fibroin microsphere and preparation thereof

Examples

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Effect test

Embodiment 1

[0023] Add 120g of silkworm seed cocoon cocoon shells to 50 times the amount of 0.1% sodium carbonate aqueous solution and boil for 30 minutes, take it out and wash it with water, repeat the above operation 1 to 2 times to ensure that all the sericin is removed, and finally degummed After the silk fibroin fiber was washed with deionized water, it was dried at 60°C for later use.

[0024] Take 10 g of the above-mentioned degummed silk fiber and dissolve it in 100 ml of calcium chloride / ethanol / water ternary solution (molar ratio is 1:2:8), and dissolve at 70° C. with constant temperature magnetic stirring. The silk fibroin solution obtained above is dialyzed, desalted and purified to obtain a water-soluble silk fibroin solution with a concentration of 2.5-3.5%, which is concentrated into a 6% water-soluble silk fibroin solution.

[0025] Take the water-soluble drug dexamethasone sodium phosphate, which accounts for 9.1% / wt of the total amount of silk fibroin, add the above-ment...

Embodiment 2

[0028] Add 120g of waste silk to 50 times the amount of 0.1% sodium carbonate aqueous solution and boil for 30 minutes, take it out and wash it with water, repeat the above operation 1 or 2 times to ensure that all the sericin is removed, and finally the degummed silk fiber After washing with deionized water, dry at 60°C for later use.

[0029]Take 20 g of the above-mentioned degummed silk fibers and dissolve them in 200 ml of calcium chloride / ethanol / water ternary solution (molar ratio is 1:2:8), and dissolve at 72° C. with constant temperature magnetic stirring. The silk fibroin solution obtained above is dialyzed, desalted and purified to obtain a water-soluble silk fibroin solution with a concentration of 2.5-3.5%.

[0030] Take 16.7% / wt of water-soluble drug vitamin C, which accounts for the total amount of silk fibroin, and add it to the above-mentioned water-soluble silk fibroin solution with a concentration of 2.5-3.5% / wt and stir evenly, then add 3% (v / v) In the liqu...

Embodiment 3

[0033] Add 60 g of waste silk to 50 times the amount of 0.1% sodium carbonate aqueous solution and boil for 30 minutes, take it out and wash it with water, repeat the above operation 1 to 2 times to ensure that all sericin is removed. Finally, the degummed silk fibers are washed with deionized water, and then dried at 60° C. for later use.

[0034] Take 10 g of degummed silk fiber and dissolve it in 100 ml of 9.0 mol / L BrLi solution, and dissolve it under constant temperature magnetic stirring at 70°C. The silk fibroin solution obtained above is dialyzed, desalted and purified to obtain a water-soluble silk fibroin solution with a concentration of 2.5-3.5%.

[0035] Take water-soluble hormone drugs accounting for 9.0% / wt of the total amount of silk fibroin, add the above-mentioned concentration of 2.5-3.5% / wt water-soluble silk fibroin solution and stir evenly, then add olive oil containing 0.8% (v / v) lecithin In 37°C, stir at 650rpm for 30 minutes at a stirring speed of 650r...

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Abstract

The invention discloses a drug-loaded microsphere and the preparation method, belonging to the technical field of biomedicine, which comprises following steps: adopting water/oil/water multiple emulsion technology and self-assembly technology of protein to fully mix the water soluble medicine and regenerated silk fibroin solution; adding the mixture in oil phase with emulsifier with certain amount during stirring for emulsification; adding organic solvent and stirring, thereby the silk fibroin is denaturalized and Beta structured to form milky crystalline silk fibroin fine particle, and the medicine is embedded; removing supernatant liquid through centrifugal effect; adding organic solvent; further crystallizing the silk fibroin and embedding the medicine; removing solvent and residual oil phase through centrifugal effect and collecting the microsphere. The average particle size of the silk fibroin drug-loaded microsphere is between 5.84 to 86.27Mum, the embedding rate is 42 to 99%, the drug loading dosage is 2.5 to 8.5%. The drug-loaded microsphere has the advantages of applicability to requirements for different purposes and wide application prospect.

Description

technical field [0001] The invention relates to a drug-loaded microsphere and a preparation method thereof, in particular to a silk fibroin drug-loaded microsphere manufactured by using silk fibroin and a preparation method thereof, which belongs to the technical field of biomedicine and also belongs to the field of polymer chemistry. Background technique [0002] Introducing drugs into the body in the form of microparticles, microcapsules, liposomes, albumin carriers, etc., can prolong the half-life of the drug, make it released slowly or at a targeted time, and improve the efficacy of the drug. The research on drug-loaded microspheres began in the mid-1970s. It is a spherical particle made of natural or synthetic polymer materials as a carrier to wrap or absorb drugs. The size of the sphere directly affects the availability of the drug in the microsphere, the drug loading of the microsphere, and the targeting of the in vivo distribution. The application fields of drug-loa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/44A61K9/16A61K47/06
Inventor 谢瑞娟吴海燕李明忠
Owner SUZHOU UNIV
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