Primer and probe of west nile virus and real time RT-PCR detection reagent kit with one-step method
A technology of RT-PCR and West Nile virus, applied in the field of real-time RT-PCR detection kit, West Nile virus RNA detection and quantitative analysis, can solve the lack of versatility, increase the cost of reagents and manpower, and reduce PCR Reaction efficiency and other issues to achieve the effect of increasing specificity and reliability of results, reducing costs and operating steps, and reducing false positive results
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[0035] Below in conjunction with specific example, further set forth the present invention. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also belong to the scope defined by the appended claims of the present application.
[0036] For the experimental methods that do not specify specific conditions in the following examples, usually follow the conventional conditions, such as: molecular cloning operation manual, or follow the conditions suggested by the manufacturer. The inorganic chemical reagents and organic solvents used meet the requirements of molecular biology experiments. Primers and probes were synthesized by Shanghai Sangon Company, pBS-T vector was purchase...
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