Primer and primer for Nipah virus, and one-step method real time RT-PCR detecting kit
A detection kit, RT-PCR technology, applied in the direction of biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the lack of versatility, increase the cost of reagents and manpower, and reduce the efficiency of PCR reactions To achieve the effect of increasing specificity and reliability of results, reducing costs and operating steps, and reducing false positive results
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[0035] The present invention will be further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.
[0036] The experimental methods that do not indicate specific conditions in the following examples usually follow conventional conditions, such as molecular cloning operation manual, or follow the conditions recommended by the manufacturer. The inorganic chemical reagents and organic solvents used meet the requirements of molecular biology experiments. Primers and probes were synthesized by Shanghai Shenggong Co., Ltd., pBS-T vector was purch...
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