Alkaline soluble lentinan extraction, separation, purification and molecular weight determination

Abstract

The invention discloses a new technology of extracting, separating and purifying 0.1-1 million Da alkali solubility lentinan and a method of molecular weight determination, by taking a mushroom fruit body as raw material, a crude product is obtained by grinding, potash leaching, filtering, concentrating, washing, grading and drying under low temperature, and then the crude product is dissolved and centrifuged, purified by a DEAE cellulose column and absorbed and discolored by ion exchange resin, and filtered to pass through membrane packets of different molecular weights for ultrafiltrating and concentrating. The molecular weight is measured by adopting a GPC laser light scattering gel chromatograph, and the obtained material is respectively put into dialysis bags for dialysis according to the measured molecular weight range, after freeze drying, the alkali solubility lentinan of various components with a molecular weight of 0.1-1 million Da and the content of more than 98 percent is obtained. The method has the advantages of advanced technology, stable quality, high purity, clear goal and easy industrialized production.

Description

Technical field The present invention is an improvement on the existing extraction process of lentinan, and discloses a new process for extraction, separation and purification of alkali-soluble lentinan and a new method for determination of molecular weight. Extracting, separating and purifying the fruit body of lentinus edodes as a raw material involves the preparation of natural biological response modifier and its detection method. Background technique Mushrooms are rich in protein, heteropolysaccharides and lentinan and other ingredients. They are traditional Chinese medicinal materials in my country and have the effect of nourishing the liver and qi. Lentinan (English name Lentinan), its primary structure is β-(1-3) glucose as the main chain and β-(1-6) glucose as the side chain glucan, which is an immunomodulatory anti-tumor assistant drug. After Chihara Goro of Japan discovered its anti-tumor activity in 1965, scientists from various countries conducted extensive resea...

AI Technical Summary

Problems solved by technology

Due to the different types of gel columns used, there is a large error in the determination of molecular weight, and it is impossible to accurately determine whether the raw material of lentinan is qualified

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