The utility model relates to the field of
flow cytometry and
fluorescence collection, in particular to a
fluorescence collection device for a
flow cytometry, which comprises a
flow cell, a collection component, a light splitting component and a detection component, the
flow cell is used for guiding and focusing the
fluorescence-labeled test particles; the collecting
assembly is used for collecting
fluorescent light and
scattered light excited by
laser irradiating the central particles of the
flow cell; the light splitting
assembly is used for dispersing multiple kinds of fluorescence collected by the collection
assembly to different receivers through the long-pass
optical filter, the dichroscope and the band-pass
optical filter, and the detection assembly is used for measuring fluorescence signals collected on the receivers. The first long-pass
optical filter is placed behind the receiving lens to suppress the short-
wavelength laser scattering light on the test particles, so that the requirement of
all optical filters on the suppression capability of short-
wavelength exciting light is reduced, and then the second long-pass optical filter is used for suppressing the long-
wavelength laser scattering light on the test particles, so that the suppression capability of
all optical filters on the short-wavelength exciting light is reduced. And the requirements of
all optical filters on the inhibition capability of the long-wavelength exciting light are reduced.