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Method for measuring molecular weight and molecular weight distribution of lentinan

A technology of molecular weight distribution and lentinan, applied in the field of biomedicine, can solve problems such as molecular weight error, and achieve the effect of improving internal quality

Inactive Publication Date: 2014-07-30
上海慈瑞医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affected by the above conditions, there is a large error between the measured result and the actual molecular weight of the substance

Method used

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  • Method for measuring molecular weight and molecular weight distribution of lentinan
  • Method for measuring molecular weight and molecular weight distribution of lentinan
  • Method for measuring molecular weight and molecular weight distribution of lentinan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Instruments: High-efficiency gel permeation chromatography is OMINI workstation system produced by Malvern Company in the United Kingdom; SHIMADZU RID-10A differential refractive index detector is produced by Shimadzu Corporation of Japan; -10AF autosampler, produced by Shimadzu, Japan; 270DUAL laser light scattering detector, produced by Malvern, UK; VE7510GPC degasser, produced by Malvern, UK. Other high-performance liquid chromatography instruments with the above-mentioned accessories and functions are also available.

[0043] (2) Chromatographic conditions:

[0044] Chromatographic column: the chromatographic column is TSK-GEL G5000PW XL Gel column, produced by Japan TOSOH company;

[0045] Mobile phase: 0.12M borate buffer solution with a pH of 9.6 was used as the mobile phase.

[0046] Flow rate: 0.5ml / min.

[0047] Injection volume: 100 μl.

[0048] Accurately weigh 25 mg of the lentinan test sample to be detected, place it in a 25ml volumetric flask, ac...

Embodiment 2

[0052] (1) Instruments: High-efficiency gel permeation chromatograph is OMINI workstation system produced by Malvern Company in the United Kingdom; SHIMADZU RID-10A differential refractive index detector is produced by Shimadzu Corporation of Japan; -10AF autosampler, produced by Shimadzu, Japan; 270DUAL laser light scattering detector, produced by Malvern, UK; VE7510GPC degasser, produced by Malvern, UK. Other high-performance liquid chromatography instruments with the above-mentioned accessories and functions are also available.

[0053] (2) Chromatographic conditions:

[0054] Chromatographic column: the chromatographic column is TSK-GEL G5000PW XL Gel column, produced by Japan TOSOH company;

[0055] Mobile phase: 0.12M borate buffer solution with a pH of 9.6 was used as the mobile phase.

[0056] Column temperature: 30°C.

[0057] Injection volume: 100 μl.

[0058] Accurately weigh 25 mg of the lentinan test sample to be detected, place it in a 25ml volumetric flask,...

Embodiment 3

[0062] (1) Instruments: High-efficiency gel permeation chromatography is OMINI workstation system produced by Malvern Company in the United Kingdom; SHIMADZU RID-10A differential refractive index detector is produced by Shimadzu Corporation of Japan; —10AF autosampler, produced by Shimadzu Corporation, Japan; 270DUAL laser light scattering detector, produced by Malvern Company, UK; VE7510GPC degasser, produced by Malvern Company, UK. Other high-performance liquid chromatography instruments with the above-mentioned accessories and functions are also available.

[0063] (2) Chromatographic conditions:

[0064] Chromatographic column: the chromatographic column is TSK—GEL G5000PW XL Gel column, produced by Japan TOSOH company;

[0065] Mobile phase: 0.12M borate buffer solution with a pH of 9.6 was used as the mobile phase.

[0066] Flow rate: 0.5ml / min.

[0067] Column temperature: 30°C.

[0068] Accurately weigh 25 mg of the lentinan test sample to be detected, place it in...

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Abstract

The invention discloses a method for measuring molecular weight and molecular weight distribution of lentinan. The method is characterized by comprising the following steps: adding lentinan to be detected into a sodium hydroxide solution for dissolving, adding a mobile phase, adding a hydrochloric acid solution for regulating the pH value, filtering, separating by utilizing a gel permeation chromatography (GPC) system, detecting by using a differential refraction detector and a laser light scattering detector, calculating the molecular weight data detected by the laser light scattering detector and concentration data detected by the differential refraction detector through GPC software so as to obtain the molecular weight and molecular weight distribution of lentinan. According to the method, the molecular weight and molecular weight distribution of lentinan can be rapidly and accurately obtained, and the method has high positive significance for improving the interior quality of the drug.

Description

technical field [0001] The invention relates to a method for measuring the molecular weight and molecular weight distribution of lentinan, which belongs to the field of biomedicine. Background technique [0002] Lentinan is a biologically active glucan extracted from Lentinus edodes, which is composed of β-(1→3) glycosidic bonds in the main chain, and the glucose groups connected by β-(1→6) are randomly distributed along the main chain A fascicular branch structure. Lentinan has inhibitory effects on various tumors, especially on digestive tract tumors. It has the advantages of low dose, good curative effect, and small toxic and side effects. [0003] The molecular weight and molecular weight distribution of lentinan are closely related to clinical antitumor activity. The study found that there are many factors that affect the biological activity of polysaccharides, such as: molecular composition, linkage mode, three-dimensional configuration, degree of branching of sugar ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/74
Inventor 朱益锋陆佳丽
Owner 上海慈瑞医药科技股份有限公司
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