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Method for increasing lincomycin yield
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A lincomycin and yield technology, applied in the biological field to achieve the effect of improving biosynthesis
Inactive Publication Date: 2008-09-17
EAST CHINA UNIV OF SCI & TECH
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However, domestic research on it mainly focuses on strain selection and breeding, while foreign counterparts' research on its fermentation process mainly focuses on the research on the synthesis pathway of lincomycin, and the research on process improvement only has the effect of cultivation temperature on lincomycin. The report on the impact of production, the impact of metal ions on the production of lincomycin has not been reported
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Embodiment 1
[0015] Embodiment 1, the impact of adding metal ions on the output of lincomycin
[0016] Prepare the fermentation medium of lincomycin, and add Na to the lincomycin fermentation medium respectively 2 MoO 4 、CoCl 2 , MnCl 2 , MgCl 2 , FeCl 3 , FeCl 2 , ZnCl 2 , CuCl 2 , so that their weight ratio concentrations in the corresponding medium are 0.001‰, 0.005‰, 0.01‰, 0.02‰ and 0.05‰, and at the same time, the original fermentation medium of lincomycin without adding metal ions is used as the control medium After inserting into Streptomyces lincolnensis (Streptomyces lincolnensis) and fermenting and culturing for 168 hours, samples were taken to determine the titer of lincomycin, and the results are shown in Table 1.
[0017] Table 1, the impact of adding metal ions on the yield of lincomycin
[0018]
[0019] According to the results in Table 1, the potency of lincomycin changed to varying degrees after adding the above-mentioned metal ions in the culture medium. 2 ...
Embodiment 2
[0020] Embodiment 2, the impact of compound adding metal ions on the output of lincomycin
[0021] 2.1. Orthogonal test of metal ions
[0022] According to the result of embodiment 1, select Na 2 MoO 4 , MgCl 2 , FeCl 3 , ZnCl 2 Carry out composite metal ion addition experiment, and take their respective optimum addition concentration as center point respectively, according to the cultivation method of embodiment 1, do four factors three levels of orthogonal experiments, investigate the interaction of each metal ion, simultaneously, with different The original fermentation medium of lincomycin added with metal ions was used as a control medium, and the yield of lincomycin in the final culture was cultured and detected, and the results are shown in Table 2.
[0023] Table 2. Table of horizontal tests added to the combination of orthogonal designs
[0024]
[0025] According to the results in Table 2, compound addition of metal ions can improve the potency of lincomycin...
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Abstract
The invention provides a method of increasing the output of lincomycin, and the method is realized through adding metal ions into a fermentation medium of the lincomycin. The method provided by the method comprises the steps that the metal ions are added into the fermentation medium of the lincomycin; whether independent metal ions are added in (for example Mg<2+>, Fe<3+> and Zn<2+> are singly added in), or complex metal ions are added in (for example the complex metal ions which are composed of Mo<6+>, Mg<2+>, Fe<3+> and Zn<2+>) to culture the lincomycin through fermentation, the biosynthesis of the lincomycin can be increased remarkably.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the yield of lincomycin. Background technique [0002] Lincomycin (LCM for short), also known as lincomycin, has a good effect on Gram-positive bacteria. It has no cross-resistance and has low toxicity, so it is widely used in At the same time, it also has a certain inhibitory effect on Gram-negative bacteria. Lincosamide antibiotics have become one of the main antibiotics in clinical application. [0003] Therefore, the research on lincomycin has become a hot spot. However, domestic research on it mainly focuses on strain selection and breeding, while foreign counterparts' research on its fermentation process mainly focuses on the research on the synthesis pathway of lincomycin, and the research on process improvement only has the effect of cultivation temperature on lincomycin. The report of the influence of production, the influence of metal i...
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