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Method for separating high quality oosperm of asropyle platichthys stellatus pallas

A technology for the separation method of flounder and flounder, which is applied in the field of artificial breeding of flounder, can solve the problems of easily spoiled water quality, reduce the quality of seedlings, and inconsistent quality of larvae, and achieve simple and easy operation methods and prevent bacteria The effect of breeding, increasing hatching rate and

Inactive Publication Date: 2008-09-24
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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AI Technical Summary

Problems solved by technology

Therefore, in the breeding process of Xingtu river flounder seedlings, if the fertilized eggs are not separated and hatched directly, the water quality will be easily damaged during the hatching process, thereby breeding bacteria and reducing the hatching rate
If the separation is not complete, the quality of the fertilized eggs collected will be uneven, the quality of the hatched larvae will be inconsistent, the growth size will vary greatly during the seedling cultivation process, and the feed specifications will not be uniform, making it difficult to carry out synchronous cultivation, thereby reducing Seed quality
[0003] Before the present invention was made, there were mainly two kinds of hatching measures taken in production for flounder: (1), after rinsing the fertilized eggs collected several times with clear water, the good and bad eggs were hatched together, and the larvae were hatched. After the film is released, the ponds are laid out. This method can remove dead eggs, but it is difficult to move the larvae and easily damage the larvae; (2). After rinsing the collected fertilized eggs with clean water, the good and bad eggs are hatched together. Lay ponds in front of the membrane, and after the larvae hatch, remove the dead eggs by sucking the bottom. This method is easy to cause bacteria to multiply and damage the larvae
[0004] At present, there is no report on the separation technology of high-quality fertilized eggs of Xingtu river flounder

Method used

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  • Method for separating high quality oosperm of asropyle platichthys stellatus pallas

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Experimental program
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Effect test

Embodiment 1

[0050] In April 2006, we conducted a separation test of high-quality fertilized eggs of Xingtu river flounder in Qingdao Longwan Biotechnology Co., Ltd. A total of 8 eggs were collected, and the egg volume was 8L. The test was divided into a control group and a 32-36‰ salinity separation group. The control group was rinsed with sand-filtered seawater for 3 times without salinity separation. The test results are shown in Table 2:

[0051] Table 2 Salinity separation results of high-quality fertilized eggs of flounder

[0052]

[0053] The test results prove that: under the same amount of eggs, the amount of high-quality fertilized eggs separated from seawater with a salinity of 32-36 is larger than that of the control group, which can effectively reduce the waste of high-quality fertilized eggs, and the fertilization rate, hatching rate and initial hatching rate are higher than those of the control group. The uniformity of the larvae was not much different from that...

Embodiment 2

[0055] From March to June 2007, we carried out the breeding of Xingtu flounder in Qingdao Longwan Biotechnology Co., Ltd. During the breeding period, a total of 23 eggs were collected, and the number of collected eggs was 14L. After being separated at a salinity of 32-36, the amount of high-quality fertilized eggs was 6L, the average fertilization rate was 87%, the hatching rate was 92%, and the hatching uniformity of larvae was 12%. %, a total of about 6 million larvae were hatched. A total of 1.372 million commercial seedlings with an average length of 5.49cm were cultivated during the acceptance by experts, and the survival rate of the seedlings was about 22.8%. The industrialized seedling breeding of Xingtu river flounder was realized and passed the acceptance of the project team.

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Abstract

A separation method for the high-quality oosperm of a platichthys stellatus pallas has the operation steps that: (1) the condign salinity range for incubating the oosperm of the platichthys stellatus pallas is confirmed to be 28 to 36 permillage; (2) the collected oosperm is added with prepared saturated and clean seawater with high salinity after being sanitized by an iodine solution, so that the salinity of the water is 32 to 41 permillage; (3) the high-quality oosperm which is floated is separated; (4) the separated high-quality oosperm is incubated after the salinity adjustment; (5) before a larval fish is incubated out of a theca, the high-quality oosperm is performed the secondary separation according to the (2) and the (3) operation steps. The operation method is simple and easy to operate, is suitable for the separation of the high-quality oosperm of the platichthys stellatus pallas, not only reduces the waste of the high-quality oosperm, but also improves the quality of the seedling. After the separation is performed by the method, the quantity of the high-quality oosperm is 6L, the average fertility rate is 87 percent, and the uniformity for incubating the larval fish is 12 percent, and the cultivated trade seedling reaches 1,372,000, the survival rate of the seedling is about 22.8 percent, and the industrial culture of seedling of the platichthys stellatus pallas is realized.

Description

technical field [0001] The invention belongs to the artificial breeding technology of flounder, and relates to a method for separating fertilized eggs of different qualities during the artificial breeding of flounder. Background technique [0002] The fertilized eggs of flounder are buoyant eggs, but because there are no oil globules in the eggs, the buoyancy of the fertilized eggs is deviated, and only some high-quality fertilized eggs are in a suspended state, while most of the high-quality fertilized eggs sink and die together. Eggs and impurities are mixed together. Therefore, in the breeding process of Xingtu river flounder seedlings, if the fertilized eggs are not separated and then hatched directly, the water quality will be easily deteriorated during the hatching process, thereby breeding bacteria and reducing the hatching rate. If the separation is not complete, the quality of the fertilized eggs collected will be uneven, the quality of the hatched larvae will be i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘长琳陈四清邹健庄志猛李学文
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI