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Fungal isolates and biological control compositions for the control of weeds

一种分离株、杂草的技术，应用在用于生物控制的化学品、使用真菌的方法、基于微生物的方法等方向，能够解决残余活性影响、没有报导过等问题

Inactive Publication Date: 2008-10-08
AGRI & AGRI FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clopyralid is used as a tillage control to achieve the same effect, but its residual activity affects some crops in the following year
[0014] Brebaum and Boland (1999, Plant Disease 83: 2000) disclosed Phoma exigua and Phoma herbarum as pathogens of dandelion (Taraxacum officinale), however, no use has been reported for These species have weed control activity when

Method used

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  • Fungal isolates and biological control compositions for the control of weeds
  • Fungal isolates and biological control compositions for the control of weeds
  • Fungal isolates and biological control compositions for the control of weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 8

[0304] In Example 8 a method for screening one or more isolates of Pheopointoma marea using random amplified polymorphic DNA (RAPD) fingerprinting is given. In this example, not intended to be limiting in any way, the primer pairs given in Table 37 can be used to amplify DNA from a number of different Phoma isolates by PCR. The PCR products can be resolved by electrophoresis. The banding pattern of isolates known to have weed control activity was found to be significantly different from that of other Phoma isolates, thus RAPD fingerprinting provides a method for screening Phoma isolates to determine whether they have weed A useful tool for controlling liveness.

[0305] Accordingly, the present invention contemplates a method of screening one or more isolates of Phomora masaurus using random amplified polymorphic DNA (RAPD) fingerprinting to determine whether the one or more isolates have weed control activity. method, said method comprising:

[0306] a) using primers selec...

Embodiment 10

[0309] A method of screening for one or more isolates of Phomasomascuum using amplified fragment length polymorphism (AFLP) is given in Example 10. In this example, not intended to be limiting in any way, the restriction enzymes EcoRI and MseI were used to digest the chromosomal DNA of a Phomoma mascara isolate to obtain a number of DNA fragments. The EcoRI and MseI sites of the DNA fragments were ligated using double-stranded oligonucleotides EcoRI and MseI linkers. The ligated fragments were then amplified by PCR using the primer pairs given in Table 38. The PCR products can be separated by electrophoresis (see Figure 21 ). The banding patterns of isolates known to have weed control activity were found to be significantly different from those of other Phoma isolates, thus AFLP fingerprinting provides a means for screening Phoma isolates to determine whether they have weed A useful tool for controlling liveness.

[0310] Accordingly, the present invention contemplates a m...

Embodiment 1

[0335] Example 1: Isolation, storage and growth of fungal isolates

[0336] 1.1 Fungal isolates

[0337] Fungal isolates were isolated from small chlorotic and necrotic spots of leaf and stem tissue collected from Canada thistle plants in fields, pastures, and roadsides. Purified fungal isolates from the plant tissue were confirmed to cause the disease symptoms using Koch's law.

[0338] Table 4. Information on fungal isolates from Canada thistle

[0339]

[0340] 1.2 Isolation of fungal isolates

[0341] Fungal isolates obtained as described above were surface sterilized in 5% sodium hypochlorite for 2 minutes, rinsed in sterile distilled water and plated on Difco PDA plates (Potato Dextrose Agar) at 24°C for 3-7 days, 12 hours of light. Fungi growing on the tissue were transferred to fresh medium and allowed to grow to maturity, respectively. Isolates were screened for pathogenicity using Koch's law and bioassays on isolated leaves. Freshly cut leaves from Canad...

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Abstract

The present invention discloses fungal isolates of Phoma macrostoma or extracts obtained therefrom, useful for the control of broad leaf weeds, including Canada thistle, perennial sowthistle, dandelion, scentless chamomile, false cleavers, chickweed, wild buckwheat, and field bindweed. The present invention also discloses biological control compositions comprising fungal isolates formulated in a growth medium for maintaining the viability of the fungal isolates when the biological control composition is applied to soil. The present invention also discloses a novel probe and primer pair sequence for use in detecting Phoma macrostoma isolates that exhibit biocontrol activity. The present invention also discloses methods of screening fungal isolates to determine if they exhibit biocontrol activity.

Description

[0001] Cross references to related patent applications [0002] This application is a continuation-in-part of U.S. application serial number 10 / 478,829, filed April 9, 2004, which is a national phase application of PCT / CA02 / 00797, filed May 20, 2002, and requires Priority is based on US Patent Application Serial No. 60 / 294,475, filed May 30, 2001. technical field [0003] The present invention relates to biological herbicides. More specifically, the present invention relates to fungal bioherbicides and compositions comprising fungal bioherbicides, and methods for screening fungal isolates to determine whether they possess biocontrol activity. Background technique [0004] The use of pesticides to kill insects, weeds and other pests is common in agriculture. It is estimated that Canadian farmers spend more than $750 million on pesticides, and it is estimated that the United States and Europe may be several times higher than Canada. On the Canadian prairie, 95 percent of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01H5/10A01P13/00C12N1/14C12P19/34C12Q1/04C12Q1/68A01N63/30
CPCC12R1/645G01N33/56961G01N2333/37G01N2500/00C12Q1/6895A01N63/04C12N1/14A01N63/30C12R2001/645C12N1/145
Inventor K·贝利J·德比
Owner AGRI & AGRI FOOD
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