Antioxidation active peptides and method for preparing same
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A technology of antioxidant active peptides and seeds, applied in the field of preparation of the antioxidant active peptides, can solve many problems such as not many
Inactive Publication Date: 2012-06-06
QIQIHAR UNIVERSITY
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Problems solved by technology
Although medical professionals have done a lot of research to solve this problem, there are not many highly active antioxidant substances that can really prevent oxidation and effectively inhibit the formation of free radicals in the body. make more efforts
Method used
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Embodiment 1
[0031] 1. Strain: Bacillus natto (Bacillus natto) strain, the deposit number is CGMCC No. 2236.
[0032] 2. Inclined medium and culture conditions:
[0033] Slant medium: beef extract 0.5g, peptone 1g, NaCl 0.5g, agar 2g, water 100mL, pH 7.0-7.2, culture at 37°C for 12 hours.
Embodiment 2
[0035] 1. Strain and slant culture are the same as in Example 1
[0036] 2. Seed culture medium and culture conditions:
[0037] Seed medium: peptone 1g, glucose 2g, K 2 HPO 4 0.1g, MgSO 4 0.05g, 100mL water, pH7.0-7.2, shaking culture for 12h at 37°C and 200r / min.
Embodiment 3
[0039] 1. Strain and slant culture are the same as in Example 1
[0040] 2. Seed cultivation is the same as in Example 2
[0041] 3. Main testing methods
[0042] (1) Protein content determination: Folin-phenol method
[0043] (2) Determination of antioxidant activity: pyrogallol auto-oxidation method
[0044] 4. Fermentation medium and fermentation conditions:
[0045] Fermentation medium: corn gluten meal and water are prepared in a certain ratio.
[0046] Take 1 mL of seed culture solution into a 250 mL Erlenmeyer flask containing 30 mL fermentation medium with an initial pH of 7.0, and shake culture for 32 h at 34°C and 200 r / min.
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Abstract
The invention discloses antioxidation bioactive peptide and a method for making the same. The product is made through the following steps that: microbial bacillus natto and corn protein powder are respectively used as strain and a raw material, which undergo liquid culture; and fermentation broth is separated through ion exchange chromatography, gel chromatography, reversed phase chromatogram and electrophoresis. The molecular weight of the product is between 20,100 and 31,000 and the five amino acid sequences at an N terminal are K-V-T-Y-H with the antioxidation activity reaching to 236.68U / mL. Moreover, the product has higher antioxidation activity.
Description
Technical field [0001] The present invention relates to an antioxidant active peptide, and the present invention also relates to a preparation method of the antioxidant active peptide. Background technique [0002] Corn Gluten Meal (CGM), commonly known as corn gluten, is one of the by-products of wet corn starch production. It contains about 60% protein. The protein composition of corn gluten meal (zein) accounts for 68%. Gluten (glutelin) accounted for 22%, and the rest are globulins and albumin. Corn gluten meal is a natural plant protein material with abundant resources and low cost. However, in terms of its amino acid composition, since the content of lysine and tryptophan is not high, corn gluten is a non-complete nutritional protein. In addition, the protein contained in corn gluten meal is difficult to dissolve in water, has a complex composition and a rough taste. Therefore, few people pay attention to it in the food field or in the feed industry, and some factories eve...
Claims
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Application Information
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