Biological tissue refractometry method based on frequency domain OCT

Abstract

The invention relates to a method for measuring the refractive index of biological tissues by utilization of the frequency-domain optical coherent tomography technology. The method is as follows: measurement is performed by utilization of the frequency-domain OCT system; interference spectrum measurement is performed for two times before and after a biological tissue sample is placed and the position of a reference mirror of a sample arm before the sample is placed and the positions of the front surface and the rear surface of the sample after the sample is placed are acquired, and then the refractive index of the sample is calculated. The sample arm of the frequency-domain OCT system is provided with a sample arm flat mirror, and the sample is attached to the flat mirror when placed. Because the frequency-domain OCT technology does not require a reference arm to perform axial scanning, the speed for measuring the refractive index under frequency-domain OCT is quicker than the measuring speed under time-domain OCT, thereby the method greatly shortens the exposure time of the sample in air, reduces the refractive index variation caused by moisture loss of the biological tissues andimproves the measurement accuracy.

Description

technical field [0001] The invention belongs to the field of biological tissue parameter measurement, in particular to a method for measuring the refractive index of biological tissue using frequency-domain optical coherence tomography technology. Background technique [0002] Refractive index is an important parameter of the optical properties of biological tissue, and has important applications in the study of light propagation in biological tissue. For highly scattering biological tissues, photons will be scattered multiple times when propagating in them, which will greatly increase the uncertainty of the angle measurement of the outgoing light. Therefore, the traditional refractive index measurement method based on the law of refraction cannot accurately measure the refractive index of complex biological tissues with multiple scattering inside. [0003] Water is an important component of biological tissue, and it makes an important contribution to the refractive index o...

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Problems solved by technology

[0006] Therefore, it can be seen from the above that time-domain OCT needs the axial scanning of the reference arm when performing refractive index measurement, and the measurement speed is limited by the axial scanning time, which cannot meet the needs of fast measurement.

This will cause changes in the refractive index of biological tissues due to the loss of water, and the measurement of the refractive index of biological tissues cannot be performed accurately and in real time.

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