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Tissue chip production method

A production method and tissue chip technology, which are applied in biochemical equipment and methods, material inspection products, and microbial measurement/inspection, etc., can solve the problems of unfavorable TMA work, inaccurate sampling sites, and deviations, etc., to achieve Avoid invalid chip deviation, technical standardization, and low cost effects

Inactive Publication Date: 2008-10-29
漆楚波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because the existing tissue microarray production methods require special instruments, which are expensive, and the sampling sites are not accurate enough, there are deviations, which is not conducive to the extensive development of TMA work.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Fabrication of tines and sampling needles. Both the punching needle and the sampling needle can be ground with a puncture needle with a solid needle. There are four needles in a set, two hollow needles and two matching solid needles. The diameter of the needle can be 0.6-2.0mm. It should be noted that the diameter of the punching needle is 1 size smaller than the diameter of the sampling needle (that is, the outer diameter of the punching needle is consistent with the inner diameter of the sampling needle), so that the diameter of the sampled tissue is consistent with the diameter of the punching hole. To prevent the connection between the tissue chip and the acceptor wax block from falling off, the diameter of the sampling needle we use is 1.6mm, and the outer periphery of the punching needle and sampling needle is covered with a colored plastic film to mark the depth of the needle entering the wax block. The depth is 1.0mm deeper than the sampling needle. It was repo...

Embodiment 2

[0013] Fabrication of flux localization templates. According to your own needs, you can use needles of different diameters to make templates with different fluxes (transparent materials are best), and the hole spacing can be 1.0mm to 2.0mm. It is recommended to use a larger spacing (2.0mm) when you are not skilled at the beginning. The distance around the blank space is a little larger (3.0mm) to prevent the punching from being crooked or cracked, which will affect the effect. There are preferably baffles around the template, preferably three weeks, and the inner diameter of the template is the same as that of the acceptor wax block, so that the template is stuck on the acceptor wax block, and the operation is very easy. There are two kinds of flux templates we use: (1) flux array 5×7, sampling diameter 2.0mm, spacing 2.0mm; (2) flux array 7×9, sampling diameter 1.5mm, spacing 1.5mm; wax block Unified as 30.0×25.0mm, thickness 15.0mm, easy to manage.

Embodiment 3

[0015] TMA preparation method, comprises the following steps

[0016] Production and punching of receptor wax blocks (1) can be made of ordinary sliced ​​pure paraffin with a melting point of 60-62°C, and various embedding frames of the same size as the template can be designed and manufactured according to one's own needs; (2) poured into the melted Add ordinary plastic embedding bottom box on the surface of the wax block to fix the wax block and number when slicing, or directly use label paper to seal the number (the bottom box and the wax block may fall off when slicing, but The wax block is still very regular and does not affect the section). Start timing after pouring liquid paraffin. After 40-50 minutes (the specific time may vary due to different room temperatures), the wax block is formed and is not completely solidified. Peel off the embedding frame and start drilling; (3) When drilling, first put The positioning template was clamped to the receptor wax block and fix...

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Abstract

The invention discloses a manufacturing method for a tissue chip, which comprises the following steps: the manufacture and perforation of a receptor wax block, the manufacture of the mark of a point to be taken and a donor wax block, the sampling of the donor wax block, recording of array point information, and the processing and slicing of an array wax block; wherein, the manufacture of the mark of the point to be taken and the donor wax block comprises the following steps: (1) when obtaining the materials, a needle is used for perforating at 1 / 3 position of the left upper quadrant of the wax block so as to mark as the origin a of an XY-axis; (2) the a is taken as the origin, and coordinate distances of the point which needs to obtain materials on the horizontal axis (X-axis) and the vertical axis (Y-axis) are respectively measured in a microscope; (3) marking is carried out when returning the corresponding coordinate point on the wax block; (4) the sampling needle is used for perforating and sampling on the parts to be taken out on the donor wax block; (5) the sample is planted accurately in the array wax block (receptor wax block); the manufacturing method of the invention can achieve the material-obtaining of precise point by point to point, thus avoiding ineffective chip deviation caused by heterogeneity of tumors reported before; the manufacturing method can also record point information of each chip precisely which is convenient for test in future, and can establish a tumor tissue chip library with normal series effectively.

Description

technical field [0001] The invention relates to a method for making a tissue chip, and in particular, the invention relates to a method for making a tissue chip capable of obtaining materials at precise locations. Background technique [0002] Tissue chip (tissue chip), also known as tissue microarray (tissue microarray, TMA), is another important biochip technology after gene chip and protein chip. This technique was first reported in Nature Medicine in 1998 by Kononen of the National Cancer Institute of the United States [1]. It is a tissue section made by arranging dozens to thousands of small tissues neatly on a glass slide according to the design. [0003] Because the existing tissue microarray fabrication methods require specialized instruments, which are expensive, and the sampling sites are not accurate enough, there are deviations, which is not conducive to the extensive development of TMA work. Contents of the invention [0004] The object of the present invent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48C12Q1/00
Inventor 漆楚波
Owner 漆楚波
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