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Devices and methods for magnetic enrichment of cells and other particles

A cell and enrichment technology, applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as insufficient enrichment of samples and rare components

Inactive Publication Date: 2008-11-12
ARTEMIS HEALTH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods often do not enrich the sample sufficiently to allow analysis of the rare components of the sample
Additionally, these techniques can lead to unacceptable losses of such rare components, for example through inefficient separation or degradation of the components

Method used

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  • Devices and methods for magnetic enrichment of cells and other particles
  • Devices and methods for magnetic enrichment of cells and other particles
  • Devices and methods for magnetic enrichment of cells and other particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0310] Embodiment 1: Silicon device of multi-channel 14 three-segment array duplex

[0311] Figure 42A -42E shows an exemplary device with the following features.

[0312] Dimensions: 90mm×34mm×1mm

[0313] Array design: three sections, the gap size of the first, second and third sections = 18, 12 and 8 μm, respectively. Bifurcation rate = 1 / 10. Diad, single bypass channel

[0314] Device design: multi-channel 14-array duplex, flow resistance device for flow stability

[0315] Device Assembly: Arrays and channels are assembled in silicon using standard photolithography and deep silicon reactive etch techniques. The etching depth is 150 μm. The via holes for fluid entry were made using KOH wet etching. The silicon substrate was sealed to the etched side using hemocompatible pressure sensitive adhesive (9795, 3M, St Paul, MN) to form an encapsulated fluid channel.

[0316] Device Packaging: The device is joined to a plastic composite tube with an external fluid reservoi...

Embodiment 2

[0323] Embodiment 2 Silicon device of multiplex 14 single-segment array duplexes

[0324] Figures 44A-44D An exemplary device is shown with the following features.

[0325] Dimensions: 90mm×34mm×1mm

[0326] Array design: 1 segment, gap size = 24 μm. Bifurcation rate = 1 / 60. Diad, dual bypass channels

[0327] Device design: multi-channel 14-array duplex, flow resistance device for flow stability

[0328] Device Assembly: Arrays and channels are assembled in silicon using standard photolithography and deep silicon reactive etch techniques. The etching depth is 150 μm. The via holes for fluid entry were made using KOH wet etching. The silicon substrate was sealed to the etched side using hemocompatible pressure sensitive adhesive (9795, 3M, St Paul, MN) to form an encapsulated fluid channel.

[0329] Device Packaging: The device is joined to a plastic composite tube with an external fluid reservoir that delivers blood and buffer to the device and extracts the resulting...

Embodiment 3

[0334] Example 3: Separation of Fetal Cord Blood

[0335] Figure 45 shows a schematic diagram of an apparatus for separating nucleated cells from fetal cord blood.

[0336] Dimensions: 100mm×28mm×1mm

[0337] Array design: three sections, the gap size of the first, second and third sections = 18, 12 and 8 μm, respectively. Bifurcation rate = 1 / 10. Diad, double bypass channel.

[0338] Device design: multi-channel 10-array duplex, flow resistance device for flow stability.

[0339] Device Assembly: Arrays and channels are assembled in silicon using standard photolithography and deep silicon reactive etch techniques. The etching depth is 140 μm. The via holes for fluid entry were made using KOH wet etching. The silicon substrate was sealed to the etched side using hemocompatible pressure sensitive adhesive (9795, 3M, St Paul, MN) to form an encapsulated fluid channel.

[0340] Device Packaging: The device is joined to a plastic composite tube with an external fluid reserv...

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PUM

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Abstract

The present invention relates to devices and methods for the enrichment of cells and other desired analytes by employing a magnetic field, alone or in conjunction with size-based separation. The devices and methods may be advantageously employed to enrich for rare cells, e.g., fetal cells or epithelial cells, present in a sample, e.g., maternal blood.

Description

Background technique [0001] The invention relates to cell separation, medical diagnostics, and microfluidic devices. Diagnostic or environmentally relevant information is often present in the sample, but in amounts too low to be detected. Therefore, various enrichment or amplification methods are often applied to increase the detectability of such information. [0002] For cells, different flow cytometry and cell sorting methods are available, but these techniques usually employ large and expensive equipment components, requiring large numbers of samples and skilled operators. These cytometers and sorters use techniques such as electrostatic deflection, centrifugation, fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) to achieve cell separation. These methods often do not enrich the sample sufficiently to allow analysis of the rare components of the sample. Additionally, these techniques can result in unacceptable losses of such rare comp...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N33/53
Inventor 拉维·卡普尔罗提恩·理查德·黄汤姆·巴伯布鲁斯·L·卡瓦略梅米特·特恩尔马丁·施密特
Owner ARTEMIS HEALTH INC
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