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Detection method of non-enzyme glucose

A detection method, glucose technology, applied in the field of glucose detection, achieves high sensitivity, large specific surface area, and improved sensitivity

Inactive Publication Date: 2008-12-10
NANJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current method for the determination of glucose is based on immobilized enzymes. Although the detection of immobilized enzymes has high selectivity and sensitivity, some shortcomings of the enzymes will appear.

Method used

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  • Detection method of non-enzyme glucose
  • Detection method of non-enzyme glucose
  • Detection method of non-enzyme glucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, the detection method of glucose,

[0040] (1) Preparation of one-dimensional mesoporous tubular Pd

[0041] a) into the device (see attached figure 1 ) by adding Cd(NO 3 ) 2 , inhale slowly with the earball, and then add the same amount of Na 2 S, inhale slowly with the suction ear ball, repeat the sample injection operation 4 to 5 times, then take out the die and dry it in a weighing bottle.

[0042] b) Electrolysis experiment:

[0043] Electrolyte: a certain amount of PdCl 2 Add HCl and sonicate for 3 minutes while stirring, add H 3 BO 3 , adjust the pH value to 6.5-7.0 with NaOH solution.

[0044] Electrolysis conditions: using a three-electrode system, the reference electrode is a saturated calomel electrode (SCE), the auxiliary electrode is platinum, and the working electrode is filled with Al 2 o 3 Template for the electrolyzer. Constant current electrolysis, the electrolysis time is 10 hours.

[0045] c) Sample detection: The sample is o...

Embodiment 2

[0050] Example 2 is basically the same as Example 1, but with the following changes: the one-dimensional mesoporous tubular Pd is changed to the one-dimensional mesoporous tubular Au.

Embodiment 3

[0051] Example 3 is basically the same as Example 1, but with the following changes: the one-dimensional mesoporous tubular Pd is changed to one-dimensional mesoporous tubular Ag.

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Abstract

The invention relates to a detecting method of glucose without enzyme, which is characterized in that the method comprises the following steps: insoluble microsphere material is added in two mutually overlapped Al2O3 templates for the space occupying; an electrodeposition method is used for electrodepositioning metal onto the Al2O3 templates; the Al2O3 templates and the space occupying material are dissolved to form mesoporous tubular metal nanometer material; mesoporous tubular metal is used for modifying an electrode; the mesoporous tubular metal is used for modifying the electrode to directly detect the glucose. The electrodepositioned metal is selected from Pd, Ag, Au or Cu. Compared with a solid tube, the detection method is provided with wider detection limit, higher sensitivity andbetter stability and reproducibility for the glucose detection. According to the experiment, the linear range of the glucose detection is 0.1-58mM, the detection limit is 0.08mM, and the stability isbetter. The method can be applied to the glucose concentration detection in clinic and environmental chemistry.

Description

technical field [0001] The invention relates to a novel, simple, rapid, sensitive and stable glucose detection method. Background technique [0002] Glucose is one of the most basic chemical building blocks in the human body and is the main source of energy for organisms. The detection of glucose with high sensitivity, high selectivity and high stability has important academic significance and application value. The current method for measuring glucose is based on immobilized enzymes. Although the detection of immobilized enzymes has high selectivity and sensitivity, some shortcomings of enzymes will appear. For example, the activity of the enzyme decreases with time, and it is volatile during the immobilization process, and is affected by temperature, humidity, pH value, and toxic substances. The proposal of an enzyme-free glucose detection method can overcome the shortcomings of the enzyme itself being unstable and easy to inactivate, and is less affected by temperature,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N27/416G01N27/327
Inventor 戴志晖包建春白红艳
Owner NANJING NORMAL UNIVERSITY
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