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Codonopsis lanceolata total saponins with antiinflammatory immunity function and preparation thereof

A technology of Codonopsis verticillum and immune function, applied in the field of medicine, can solve the problems of no patent application and no report on the application of total saponins of Codonopsis verticillum, and achieve the effect of simple preparation process and clear mechanism of action

Inactive Publication Date: 2008-12-17
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application Nos. 02109559.0 and 200510136742.8 respectively disclose invention patents named "blood fat-lowering health drink and its preparation method" and "codonopsis codonopsis health drink", but no other patent applications have been seen
At present, there is no report on the application of Codonopsis saponins in the treatment of inflammatory and immune diseases

Method used

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  • Codonopsis lanceolata total saponins with antiinflammatory immunity function and preparation thereof
  • Codonopsis lanceolata total saponins with antiinflammatory immunity function and preparation thereof
  • Codonopsis lanceolata total saponins with antiinflammatory immunity function and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Take 5kg of Codonopsis radix codonopsis coarse powder, add 35L of 70% ethanol for soaking, heat and reflux for extraction 3 times, the extraction time is 2, 2, and 1 hour respectively, combine the alcohol extracts, filter, and concentrate under reduced pressure at 60 ° C until the solution contains The concentration of crude drug is 30g / ml, filtered; the filtrate is adsorbed by macroporous adsorption resin, the amount of resin is 10kg, the impurities are washed with water and 20% ethanol respectively, and then eluted with 75% ethanol, the alcohol eluent is collected, and the pressure is reduced. The solvent was recovered, the obtained crude product was suspended in an aqueous solution, extracted with n-butanol, and the solvent was recovered under reduced pressure to obtain 281 g of the total saponins.

[0035] The general structural formula is:

[0036]

Embodiment 2

[0038]Take 5kg of North American ginseng coarse powder, add 35L of 70% ethanol for soaking, heat and reflux for extraction 3 times, the extraction time is 2, 2, and 1 hour, respectively, combine the extracts, filter, filter, and concentrate under reduced pressure at 60 ° C to a solution The concentration of the crude drug contained is 40g / ml, and it is filtered; the filtrate is adsorbed by macroporous adsorption resin, and the amount of resin is 8kg. The impurities are washed with water and 20% ethanol respectively, and then eluted with 70% ethanol solution, and the alcohol eluent is collected, The solvent was recovered under reduced pressure, the obtained crude product was suspended in an aqueous solution, extracted with n-butanol, and the solvent was recovered under reduced pressure to obtain 264 g of the total saponins.

Embodiment 3

[0039] Example 3 Inhibitory effect of total saponins from Codonopsis radix on xylene-induced ear swelling in mice

[0040] (1) Experimental materials

[0041] Animals: Male KM mice (25±2g), fasted for 24 hours before administration, and had free access to water.

[0042] Reagents: physiological saline, xylene, 0.5% CMCNa, aspirin (Shenyang Wanrong Pharmaceutical Co., Ltd., product batch number: 061205), indomethacin (Shanxi Yunpeng Pharmaceutical Co., Ltd., product batch number: A070801) ).

[0043] Materials and instruments: 1ml mouse gavage device, 1ml syringe, surgical scissors, tweezers, punch, volumetric flask, TG328A(S) analytical balance (Shanghai Jingke Instrument Factory).

[0044] (2) Experimental method

[0045] Mice were randomly divided into 5 groups with 10 mice in each group: negative control group (0.2% CMCNa, 10ml / kg, i.g.), aspirin control group (30mg / kg) -1 , i.g.), indomethacin control group (10mg / kg -1 , i.g.), drug group (30, 20 mg / kg, i.g.). 30 min...

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Abstract

The invention belongs to the technical field of medicine and provides a method for preparing a traditional Chinese medicine total saponin of codonopsis lanceolata; the total saponin is obtained mainly by refluxing extraction of the codonopsis lanceolata with 70% of ethanol, absorption with macroporous adsorbent resin, removal of impurities by washing with water, elution with alcoholic liquid and extraction of the eluent with n-butyl alcohol. The total saponin mainly comprises seven effective triterpenoid saponin active ingredients such as codonolaside, codonolaside I, codonolaside II, codonolaside III, codonolaside IV, eclalbasaponin XIII, and echinocystic acid 3-O-Beta-D-glucurono-pyranoside, etc. Test results show that the total saponin of codonopsis lanceolata has obvious anti-inflammation and immunoregulation effects. The total saponin has definite material foundation and mechanism of action; the preparation process is simple and suitable for industrial production. The total saponin can be taken as pharmaceutical raw material for preparing medicines for treating inflammation and immunological diseases such as rheumatoid arthritis, chronic bronchitis, systemic lupus erythematosus, and the like, and can be made into clinically acceptable dosage forms such as tablets, capsules, granules or compound preparation, and the like, according to the conventional process.

Description

technical field [0001] The invention relates to the technical field of medicine, relates to total saponins of Codonopsis radix and a preparation method thereof, and also relates to the medicinal use of total saponins of Codonopsis radix for treating inflammatory immune diseases. Background technique [0002] Inflammatory and immune diseases, such as rheumatoid arthritis, chronic bronchitis, systemic lupus erythematosus, etc., are clinically common chronic diseases characterized by recurrent chronic processes. It involves various mechanisms such as immune dysfunction. At present, the treatment of rheumatoid arthritis mainly focuses on anti-inflammatory and analgesic; the treatment of chronic bronchitis mainly focuses on the research on anti-inflammatory, antibacterial, antitussive and antiasthmatic effects. drug. One of the most important reasons for the pathogenesis of inflammatory immune diseases is the immune dysfunction of the body. Patients repeatedly use broad-spectru...

Claims

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Application Information

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IPC IPC(8): A61K36/344A61K31/704A61P11/00A61P19/02A61P29/00A61P37/02C07J63/00A61K125/00
Inventor 原忠梁志敏徐丽萍张莉萍
Owner SHENYANG PHARMA UNIVERSITY
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