Rotavirus and ad virus two-in-one detection kit

A technology of rotavirus and adenovirus, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of costing materials, labor and time, and achieve the effect of convenient use

Inactive Publication Date: 2009-01-07
深圳市惠安生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] It can be seen that when analyzing the etiology of children's diarrhea in the prior art, two tests are usually re...

Method used

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Embodiment Construction

[0015] The present invention is described in detail below.

[0016] The two-in-one detection box for rotavirus and adenovirus of the present invention is mainly used for simultaneous detection of rotavirus and adenovirus in human feces by using color polymer latex labeling immunochromatography, and belongs to clinical in vitro diagnostic reagents.

[0017] Non-colloidal gold polymer microspheres exist in a monodisperse phase, and the prepared marking liquid is uniformly dispersed, easy to spray, easy to flow, easy to observe, and has high sensitivity; moreover, it also has different molecular weights, different structural forms and Surface characteristics and polymer microspheres with different functional groups introduced on the surface; it has the advantages of large specific surface, strong adsorption, high mechanical properties and excellent solvent resistance, convenient recycling and reuse, and is easy to mix with antibodies (antigens) ) connection; this kind of colored ...

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Abstract

The invention discloses a dual detection box of rotavirus and adenovirus and comprises test objects and feces diluents; wherein, the test objects consist of chromatographic films and water absorbent materials and comprise a first area, a second area, a third area and a fourth area; the first area comprises an absorbent pad made of water absorbent materials and used for initial sampling contact; the second area comprises latex particles which are coated by monoclonal antibodies of the rotavirus and adenovirus; the third area comprises three lateral parallel chromatographic films which can not be seen with naked eyes, and a first film is provided with a specific anti-adenovirus antibody, and a second film is provided with a specific anti-rotavirus antibody and a third film is a quality control line; the fourth area comprises an absorbent pad made of the water absorbent materials. The detection box is convenient in use and can be used for detecting the acute diarrhea, vomiting, high fever and stomachache which are caused by viral gastroenteritis of adults and infants with less time, labor and materials.

Description

technical field [0001] The present invention relates to a substance used in the testing and analysis of biological substances, in particular to a detection box used in the immunological detection of biological substances. Background technique [0002] Rotavirus is the main cause of acute gastroenteritis, especially in children under 2 years old. About 40% of children with gastroenteritis can detect rotavirus in their feces. Gastroenteritis caused by enteroviruses can be fatal in at-risk groups such as children, the elderly, or immunosuppressed people. In warmer climates, rotavirus infection usually occurs during the winter. Their transmission is the oral-fecal route, with an incubation period of 1-3 days, typical symptoms include vomiting, 3-8 days of watery diarrhea, high fever, and stomach pain, after the first infection, immunity is incomplete, but subsequent infections Often less serious than the first time. Rotavirus is also the most common cause of acute diarrhea. A...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
Inventor 高翔
Owner 深圳市惠安生物科技有限公司
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