Enhancement of growth in plants
A plant growth and plant technology, applied in the direction of plant growth regulators, plant genetic improvement, botany equipment and methods, etc., can solve the problems of biological research difficulties, mycorrhizal fungi have not obtained the frequency of nodulation bacteria, inconsistency, etc.
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Embodiment 1
[0355] Example 1 - Effect of Treatment of Tomato Seeds with an Allergic Response Elicitor of Erwinia amylovora on Germination Rate
[0356] Seeds of Marglobe var. tomato were soaked in 40 ml Erwinia amylovora hypersensitive response elicitor solution ("harpin"). Harpin was prepared as follows: Cultures containing plasmid pCPP2139 (see figure 1 ) Escherichia coli strain DH5, the cells were lysed by sonication, kept in boiling water for 5 minutes for heat treatment, centrifuged to remove cell debris, and then precipitated proteins and other heat-labile components. The resulting preparation ("CFEP") was serially diluted. These dilutions (1:40, 1:80, 1:160, 1:320, and 1:640) contained 20, 10, 5, 2.5, and 1.25 μgm / ml of harpin, respectively, according to Western blot analysis. On day 0, seeds were soaked in harpin or buffer in beakers for 24 hours at 28°C in a growth chamber. After soaking, the seeds were sown on day 1 in germination pots filled with artificial soil. This proc...
Embodiment 2
[0367] Example 2 - Effect of treatment of tomato seeds with Erwinia amylovora hypersensitivity elicitor on tomato plant height
[0368] On day 0, in a growth chamber, seeds of Marglobe var. tomato were soaked in beakers with Erwinia amylovora harpin (1:15, 1:30, 1:60 and 1:120) or buffer 24 hours in solution. After soaking, the seeds were sown on day 1 in germination trays with artificial soil.
[0369] Ten consistent plants were randomly selected for each treatment and measured. Use a ruler to measure the height of the seedlings from the soil surface to the top of the plant.
[0370] deal with:
[0371] 1. Harpin (1:15) (52 μgm / ml).
[0372] 2. Harpin (1:30) (26 μgm / ml).
[0373] 3. Harpin (1:60) (13 μgm / ml).
[0374] 4. Harpin (1:120) (6.5 μgm / ml).
[0375] 5. Buffer (5mM KPO 4 , pH 6.8).
[0376]
[0377]
[0378] Table 5 - Summary - Average height of tomato plants after treatment
[0379]
[0380] As shown in Tables 2-5, treatment of tomato seeds with Er...
Embodiment 3
[0381] Example 3 - Effect of treating tomato plants with Erwinia amylovora hypersensitivity elicitor on tomato plant height
[0382] When Marglobe tomato plants are 4 weeks old, spray each plant with 6 ml of Erwinia amylovora harpin containing 13 μgm / ml (1:60) or 8.7 μgm / ml (1:90) harpin in a growth chamber at 28°C solution or spray 6ml buffer (5mM KPO 4). The height of the tomato plants was measured 2 weeks after spraying harpin (6 week old tomato plants) and 2 weeks and 5 days after. Ten consistent plants were randomly selected for each treatment and measured. Use a ruler to measure the height of the seedlings from the soil surface to the top of the plant.
[0383] deal with:
[0384] 1. Harpin (1:60) (13 μgm / ml).
[0385] 2. Harpin (1:90) (8.7 μgm / ml).
[0386] 3. Buffer (5mM KPO 4 , pH 6.8).
[0387] Table 6-Average height of tomato plants after harpin treatment
[0388]
[0389] As shown in Table 6, spraying tomato seedlings with Erwinia amylovora hypersensiti...
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