Enhancement of growth in plant
A plant growth and plant technology, applied in the fields of plant growth regulators, plant regeneration, plant genetic improvement, etc., can solve the problems of biological research difficulties, mycorrhizal fungi have not obtained the frequency of use of nodulating bacteria, inconsistency, etc.
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Embodiment Embodiment 1
[0268] EXAMPLES Example 1 - Effect of treatment of tomato seeds with an allergic reaction elicitor of Erwinia amylovora on germination
[0269] rate impact
[0270] Seeds of Marglobe var. tomato were soaked in 40 ml Erwinia amylovora hypersensitive response elicitor solution ("harpin"). Harpin was prepared as follows: Cultures containing plasmid pCPP2139 (see figure 1 ) Escherichia coli strain DH5, the cells were lysed by sonication, kept in boiling water for 5 minutes for heat treatment, centrifuged to remove cell debris, and then precipitated proteins and other heat-labile components. The resulting preparation ("CFEP") was serially diluted. These dilutions (1:40, 1:80, 1:160, 1:320, and 1:640) contained 20, 10, 5, 2.5, and 1.25 μgm / ml of harpin, respectively, according to Western blot analysis. On day 0, seeds were soaked in harpin or buffer in beakers for 24 hours at 28°C in a growth chamber. After soaking, the seeds were sown on day 1 in germinati...
Embodiment 2
[0281] As shown in Table 1, treatment of tomato seeds with Erwinia amylovora hypersensitivity elicitors reduced the time required for germination and greatly increased the germination rate. Example 2 - Effect of treatment of tomato seeds with Erwinia amylovora hypersensitivity elicitor on tomato plants
[0282] high impact
[0283] On day 0, in a growth chamber, seeds of Marglobe var. tomato were soaked in beakers with Erwinia amylovora harpin (1:15, 1:30, 1:60 and 1:120) or buffer 24 hours in solution. After soaking, the seeds were sown on day 1 in germination trays with artificial soil.
[0284] Ten consistent plants were randomly selected for each treatment and measured. Use a ruler to measure the height of the seedlings from the soil surface to the top of the plant.
[0285] Treatment: 1. harpin (1:15) (52 μgm / ml). 2. Harpin (1:30) (26 μgm / ml). 3. Harpin (1:60) (13 μgm / ml). 4. Harpin (1:120) (6.5 μgm / ml). 5. Buffer (5mM KPO 4 , pH6.8).
[0286] deal with...
Embodiment 5
[0328] As shown in Tables 7-10, treatment of tomato seeds with an Erwinia amylovora hypersensitive response elicitor enhanced tomato plant growth. The 1:160 dilution (5 μg / ml harpin) had the greatest effect - seedling height increased by more than 20% over buffer-treated plants. Example 5 Treatment of tomato seeds with Erwinia amylovora hypersensitivity elicitor vs. seed
[0329] The effect of germination
[0330] On day 0, Marglobe tomato seeds were soaked in a beaker at 28°C in 40 ml of Erwinia amylovora hypersensitive response elicitor ("harpin") solution (from CFEP of E. coli DH5(pCPP2139)) in a growth chamber. 1:50 or 1:100 dilutions containing 8 μgm / ml and 4 μgm / ml of the hypersensitivity elicitor, respectively) or in buffer for 24 hours. After soaking, the seeds were sown on day 1 in germination trays with artificial soil. Each treatment was performed in 4 pots with 20 seeds in each pot. Treatment: 1. harpin (8 μgm / ml). 2. Harpin (8 μgm / ml). 3. Harpin (8 μgm / ml). ...
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