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Preparation method for chitosan oligosaccharide

A technology of chitosan oligosaccharide and chitosan is applied in the field of preparation of chitosan oligosaccharide products, which can solve the problems of rare reports of chitosan oligosaccharide and wide molecular weight distribution, so as to improve the immune effect, reduce the inhomogeneity, and speed up the development of the chitosan oligosaccharide. response rate effect

Inactive Publication Date: 2009-01-14
BEIJING JIANERKANG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although there are so many patents and research reports, as well as functional products listed by manufacturers, their products have a wide molecular weight distribution, especially reports on chitosan oligosaccharides with hexaoligosaccharides as the main body are rare.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 60g of chitosan powder (purchased in the market) is stirred and dissolved with 2000ml of 3wt% acetic acid solution, 50mg of chitosan A enzyme is added, the mixture is put into a microwave field, and the microwave power is 1000W, and the temperature is raised to 48°C for compounding After 30 minutes of degradation reaction, the resulting product was taken out from the microwave field, firstly subjected to microporous filtration, then filtered through a membrane of 10,000-20,000 mesh molecular sieves, concentrated in a vacuum at 45°C, and dried by a spray dryer to obtain the chitosan oligosaccharide product, which is light yellow in color and heavy in weight. The average molecular weight is 1000, the degree of deacetylation is 80%, it is completely soluble in water, and the recovery rate is 90%.

Embodiment 2

[0015] Chitosan powder 100g (purchased in the market) is stirred and dissolved with 2000ml of acetic acid solution with a concentration of 5wt%, and 300mg of chitosan A enzyme is added, the mixture is put into a microwave field, and the microwave power is 1000W, and the temperature is raised to 40°C for compounding After 15 minutes of degradation reaction, the resulting product was taken out from the microwave field, firstly subjected to microporous filtration, then filtered through a molecular sieve membrane of 10,000 to 20,000 mesh, concentrated in vacuum at 50°C, and dried by a spray dryer to obtain the chitosan oligosaccharide product, which is light yellow in color and heavy in weight. The average molecular weight is 2000, the degree of deacetylation is 82%, it is completely soluble in water, and the recovery rate is 90%.

Embodiment 3

[0017] Chitosan powder 100g (purchased in the market) is stirred and dissolved with 2000ml of acetic acid solution with a concentration of 5wt%, and chitosan A enzyme 100mg is added, the mixture is put into a microwave field, and the microwave power is 1000W, and the temperature is raised to 40°C for compounding After 30 minutes of degradation reaction, the obtained product was taken out from the microwave field, firstly subjected to microporous filtration, and then filtered through a membrane of 10,000-20,000 mesh molecular sieves, concentrated in vacuum at 50°C, dried by a spray dryer, and further passed through 100 mesh molecular sieves to obtain the color of the product Light yellow, weight-average molecular weight 3000, degree of deacetylation 85%, completely soluble in water, recovery rate 92%.

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Abstract

The invention relates to a preparation method of using microwave enzyme to prepare chitosan oligosaccharide product with the small molecular weight taking hexa-oligosaccharide as the main body in a compound way, which belongs to the biochemistry field of the chitosan oligosaccharide product preparation from chitosan. Chitosan material is dissolved with acetic acid, the weight percentage of the concentration of chitosan in the acetic acid solution occupies 2 percent to 7 percent, after chitosan A enzyme is added, the weight ratio of the chitosan A enzyme and the chitosan zymolyte occupies 0.05 percent to 0.5 percent; the mixture is put in the microwave field, the power of the microwave field is 1000 W to 2000 W, compound degradation reaction is performed when the temperature is raised at 30 DEG C to 60 DEG C, and after the reaction is finished, firstly millipore filtration is conducted, afterwards the film of a molecular sieve is used for filtration, and after the working procedures of low temperature vacuum concentration and spray drying, the yellowish chitosan oligosaccharide product is obtained, the weight average molecular weight between 500 to 5000 can be controlled, the hexa-oligosaccharide proportion is high, the deacetylation degree is 80 percent to 90 percent, and the recovery ratio is 90 percent to 95 percent.

Description

technical field [0001] The invention belongs to the field of biochemical industry for preparing chitosan oligosaccharide products from chitosan, in particular to a method for preparing low molecular weight chitosan oligosaccharide products mainly composed of hexaoligosaccharides by using microwave-enzyme compounding. Background technique [0002] Chitin is a renewable resource second only to cellulose in reserves on the earth. Chitosan is its deacetylated derivative. Chitosan is not only rich in sources but also has many physiological activities, which can reduce cholesterol and blood lipids and enhance immunity. It also has the effects of antibacterial, antitumor, and regulating plant growth. However, due to the large molecular weight of chitosan, it is limited to be absorbed by the human body, animals and plants, which limits its efficacy. Therefore, the preparation of chitosan oligosaccharides with small molecular weight has become a hot spot in this field. [0003] The ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
Inventor 郑宁杨军
Owner BEIJING JIANERKANG BIOTECH DEV