Method for preparing total free anthraquinones extract from rhubarb

A technology of rhubarb total anthraquinone and rhubarb extract, which is applied in the field of medicine and can solve problems such as cumbersome operation

Inactive Publication Date: 2009-01-21
石任兵
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the preparation method of rhubarb free anthraquinone, see the published invention patent 99114211.X, the preparation of rhubarb free anthraquinone with ion exchange resin is cumbersome to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Preparation process of rhubarb total free anthraquinone extract

[0011] Take 1 kg of the rhubarb extract, add 5 times the amount of water to disperse and dissolve, centrifuge at a speed of 3000 rpm for 30 minutes to obtain a precipitate, dissolve it with 30% ethanol solution and put it on a polyamide column. The concentration of the sample solution is 3.63 mg / min. mL (based on the total anthraquinone content), the adsorption flow rate is 2.0mL / min, the diameter-to-height ratio is 1:8, the sample loading is 11.3 mg / mL (based on the total anthraquinone content), and 50% ethanol elutes 5 times the column Volume for impurity removal, impurity removal flow rate is 1.0mL / min, 90% ethanol elution column volume, elution flow rate is 2.0mL / min, collect 90% ethanol eluate, recover solvent, dry under reduced pressure, namely Total free anthraquinone sites. The total anthraquinone content was determined to be 52.58%, and the five components of aloe-emodin, rhein, emodi...

Embodiment 2

[0012] Example 2: Preparation process of rhubarb total free anthraquinone extract

[0013] Take 1kg of rhubarb extract, add 5 times of water to disperse and dissolve, centrifuge at 3000 rpm for 30min to obtain the precipitated part, dissolve it with 30% ethanol solution and put it on the polyamide column, the concentration of the sample solution is 4.00mg / mL (Based on total anthraquinone content), adsorption flow rate is 2.0mL / min, diameter-to-height ratio is 1:6, sample loading is 9.36mg / mL (calculated on total anthraquinone content), eluted with 50% ethanol for 6 column volumes Carry out impurity removal, impurity removal flow rate is 1.0mL / min, 90% ethanol elution 7 times column volume, elution flow rate is 2.0mL / min, collect 90% ethanol eluate, recover solvent, dry under reduced pressure, namely total Free anthraquinone sites. The total anthraquinone content was determined to be 53.89%, and the five components of aloe-emodin, rhein, emodin, chrysophanol and emodin methyl ...

Embodiment 3

[0014] Example 3: Preparation process of rhubarb total free anthraquinone extract

[0015] Take 1kg of rhubarb extract, add 5 times of water to disperse and dissolve, centrifuge at 3000 rpm for 30min to obtain the precipitated part, dissolve it with 30% ethanol solution and put it on the polyamide column, the concentration of the sample solution is 4.13mg / mL (Based on total anthraquinone content), adsorption flow rate is 2.0mL / min, aspect ratio is 1:7, sample loading is 10.45 mg / mL (calculated on total anthraquinone content), eluted with 50% ethanol for 7 times column volume Carry out impurity removal, impurity removal flow rate is 1.0mL / min, 90% ethanol elution 8 times column volume, elution flow rate is 2.0mL / min, collect 90% ethanol eluate, recover solvent, dry under reduced pressure, namely total Free anthraquinone sites. The total anthraquinone content was determined to be 53.71%, and the five components of aloe-emodin, rhein, emodin, chrysophanol and emodin methyl ether...

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PUM

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Abstract

The invention discloses a method for extracting rhubarb total free anthraquinone extract from rhubarb. The content of total anthraquinone in the rhubarb total free anthraquinone extract is 5-100% (w / w), wherein, the content of five components, namely, rheum emodin, rhein, aloe-emodin, chrysophanol and physcion, accounts for 5-100% (w / w) of all content of total anthraquinone. The method includes the following steps: 1. rhubarb extract is added with acid and then heated for hydrolyzing, and hydrochloric acid is the preferred acid. 2. hydrolysate solution is centrifugated, precipitation part is dissolved by ethanol and put on polyamide columns, effluent liquid is removed, ethanol with a certain concentration is used for removing impurities, finally methanol or ethanol is used for eluting, eluate is collected and methanol or ethanol is removed by evaporation to obtain the rhubarb total free anthraquinone extract which essentially contains rheum emodin, rhein, aloe-emodin, chrysophanol and physcion.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method of a total anthraquinone extract of rhubarb, a traditional Chinese medicine. Background technique [0002] Rhubarb is a commonly used traditional Chinese medicine, which is derived from the dried roots and rhizomes of Rheum palmatum L., Rheum tanguticum Maxim.ex Balf. or medicinal rhubarb (Rheum officinale Baill.) of Polygonaceae. Traditional Chinese medicine theory believes that rhubarb has multiple functions such as purging heat and dredging intestines, cooling blood and detoxifying, expelling blood stasis and dredging menstrual flow, etc. Swelling, intestinal carbuncle abdominal pain, carbuncle swollen furuncle, blood stasis amenorrhea, injury from falling, external treatment of water and fire burns and other syndromes. Modern pharmacological studies have shown that rhubarb has anti-pathogenic microorganisms, anti-inflammation, anti-tumor, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61P31/00A61P29/00A61P35/00A61P3/06A61P3/10A61P9/10A61P1/16A61P13/12A61P39/06A61P37/02A61P1/10A61P17/02A61K125/00
Inventor 石任兵张小茜刘芬娣刘斌姜艳艳
Owner 石任兵
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