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Use of amaryllidaceae alkaloid as tumour anti-apoptosis factor Mc1-1 inhibitor

An alkaloid and anti-apoptosis technology, applied in the field of medicine, can solve problems such as treatment failure

Inactive Publication Date: 2009-01-28
SHANTOU UNIV MEDICAL COLLEGE
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  • Abstract
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Problems solved by technology

However, in clinical treatment, tumor cells are often resistant to most of the above-mentioned drugs, leading to treatment failure.

Method used

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  • Use of amaryllidaceae alkaloid as tumour anti-apoptosis factor Mc1-1 inhibitor
  • Use of amaryllidaceae alkaloid as tumour anti-apoptosis factor Mc1-1 inhibitor

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Embodiment Construction

[0018] Lycorine preparation and cell culture

[0019] A storage solution with a concentration of 10 mM lycorine was prepared with sterile saline. K562 cells, K562 drug-resistant cells, and primary cultured acute myeloid leukemia cells were cultured conventionally (RPMI-1640 medium containing 10% calf serum, 100 u / ml of penicillin and streptomycin) at 37°C, Saturated humidity, 5% CO 2 Cultured in an incubator, and the medium was changed every 3 to 4 days. Collect logarithmic growth phase cells, adjust the number of cells to 1×10 5 / ml, take 10mL to the culture dish, add lycorine with the final concentration of 1, 5, 10uM respectively to act on K562 cells and K562 drug-resistant cells for 24 hours, except that the control group does not add lycorine, and the other conditions are exactly the same . Incubate at 37°C and collect cells.

[0020] Lycorine inhibits cell proliferation activity

[0021] MTT purchases Sigma Corporation. Prepare a 5 mg / ml stock solution with deioni...

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Abstract

The invention belongs to the field of medicine, which relates to the application of Lycoris radiate alkaloid that is taken as tumor anti-apoptosis factor Mcl-1(myeloid cell leukemia-1) inhibitor, by inhibiting the protein expression of the tumor anti-apoptosis factor Mcl-1(myeloid cell leukemia-1), the diseases that are caused by the protein expression exception of Mcl-1, such as autoimmune inflammations, tumors, and the like, can be cured.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the application of Lycoris alkaloids as tumor anti-apoptosis factor Mcl-1 (myeloid cell leukemia-1) inhibitors. technical background [0002] Lycorine is one of the main alkaloids present in Chinese medicinal materials Lycoris or Narcissus root. It is insoluble in water, but its hydrochloride is soluble in water. Lycoris is the bulb of the Amaryllidaceae plant. It is produced in most parts of my country. It has the effects of repelling phlegm, diuresis, detoxification, and emetic. Narcissus root is the bulb of the same family plant Narcissus, which is mainly distributed in southeast my country and other places. It is mainly used to treat carbuncle sores, insect bites, and fish bones. It has been confirmed that the above-mentioned medicinal effects are mainly attributed to various active ingredients of alkaloids in the medicinal materials, mainly lycorine. Lycorine has been used as an e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/437A61K31/407A61P35/00
Inventor 蒋纪恺刘小珊
Owner SHANTOU UNIV MEDICAL COLLEGE
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