Double antigen sandwich method for detecting antibody by indirectly marked nanometer granule and kit thereof

Abstract

The invention provides an antibody detection double-antigen sandwich method using nanoparticles. A nanoparticle label and a label between the labeled antigens implement an indirect labeling by combining the label on an antigen and a ligand which is labeled on the nanoparticle label and can specifically recognize the label, wherein the labeled antigen is a gene engineering recombined antigen. Withthe indirect labeling manner, the sensitivity and the specificity of the method can be remarkably improved.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to an antibody detection double-antigen sandwich method of indirectly labeling nanoparticles, and an immunoassay kit prepared by the method. Background technique [0002] For the detection of the target antibody in the specimen, immunological methods can be used for detection. Several methods that are widely used now are: radioimmunoassay, immunofluorescence, ELISA, etc., but they all have shortcomings and deficiencies in varying degrees. [0003] The radioimmunoassay has radioactive contamination, and the detection time is long, requiring professional equipment and professionals to operate, and single-person detection cannot be realized; the detection time of the immunofluorescence method is long, requires professional equipment and professional operation, and is easily interfered by interfering substances to produce false positives. Positive; Enzyme-linked immunosorbent assay (ELISA) h...

AI Technical Summary

Problems solved by technology

[0009] 3. Since the labeled antigen generally contains a certain amount of miscellaneous proteins, the miscellaneous proteins are also labeled on the marker together with the target protein during the labeling process, resulting in a decrease in the specificity of the labeling complex;

[0010] 4. Conditions such as buffer solution, pH, and ionic strength for direct labeling cannot guarantee the optimal activity conditions of the labeled antigen. Factors such as vigorous stirring, centrifugation, and blocking during labeling are also likely to affect the active epitope of the labeled antigen, resulting in Reduced sensitivity of final detection

[0013] In summary, the markers used in the double-antigen sandwich detection kit for antibody detection based on recombinant antigen nanoparticles are currently using direct labeling, which has methodological defects. Therefore, further research is urgently needed on this basis. Improved, increased kit detection sensitivity and improved specificity

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