Rice tillering associated protein and encoding gene and use thereof

A technology of tiller-related proteins and coding genes, which is applied in the field of rice tiller-related proteins and their coding genes and applications, can solve the problems of only increasing the number of tillers, rice multi-tiller mutants, etc., and achieve the purpose of increasing rice yield and maximizing economic output and avoid the effect of wasting nutrients

Inactive Publication Date: 2009-03-25
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

So far, there has been no report of rice multi-tiller mutan

Method used

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  • Rice tillering associated protein and encoding gene and use thereof
  • Rice tillering associated protein and encoding gene and use thereof
  • Rice tillering associated protein and encoding gene and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the acquisition of rice tiller-related protein and its coding gene

[0046] 1. Genetic analysis of rice tiller-related gene HTD1

[0047] The multi-tiller dwarf mutant Xintai'ai (indica rice) was isolated from a cross between the double dwarf mutant Aitaiyin-2 and the high-stalk indica variety Nanjing 6 (Liang GH, P., X.B., Gu, M.H., Ji , C.Q.(1995). "The isolation and genetic identification of a semidwarf gene from an indicarice variety Aitaiyin 2." Chinese J.RICE Sci .9:189-192). After the rice plants have been headed and the height has been determined, the orthogonal and reciprocal F 1 The plant height and tiller number of generation, the results are shown in Table 1, show that the plant height of the multi-tiller dwarf mutant Xintaiai is significantly lower than the wild type variety Nanjing 6 (82.64 / 140.52), and the decline rate is 41.2%; The number of tillers in Tai'ai was significantly higher than that of the wild-type variety Nanjing 6 (99.5 / 11...

Embodiment 2

[0072] Embodiment 2, expression analysis of HTD1

[0073] In order to study the expression pattern of HTD1 in rice, RNA from different tissues such as rice leaves, stems, panicles and roots were extracted, and Northern hybridization was carried out with HTD1 cDNA (sequence 2) as a probe. The results are as follows Figure 8 As shown, it shows that HTD1 is expressed in the above tissues, but the expression in the aerial parts is stronger, and the expression in the root is weaker. In addition, in the transgenic plants obtained by transforming rice with the vector containing HTD1 and GUS fusion protein encoding gene HTD1::GUS, the results of GUS histochemical staining showed that the midrib, leaf sheath, and stem node of rice plants with HTD1::GUS gene, GUS was expressed in panicle and root tissues, and each tissue further confirmed the Northern result of HTD1 gene expression pattern; at the same time, the results of GUS histochemical staining also revealed that HTD1 gene may onl...

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Abstract

The invention discloses a protein relative to rice tillering as well as a coding gene and the application thereof. The protein relative to rice tillering is the protein with one of the following amino-acid residue sequences: (1) SEQ ID NO: 3 in a sequence list; (2) the protein, in which the SEQ ID NO: 3 in the sequence list is replaced, deleted or added by one or a plurality of amino-acid residues, and which is correlated with the rice tillering. The protein relative to rice tillering and the coding gene thereof play an important role in the reasonable configuration of the rice plant structure and the further rice yield improvement.

Description

[0001] This application is a divisional application with the application number 200510059808.8, the application date is March 31, 2005, and the invention title is "rice tillering-related protein and its coding gene and application". technical field [0002] The invention relates to a rice tiller-related protein, its coding gene and application. Background technique [0003] Rice is one of the most important food crops in the world, and more than half of the world's population uses rice as the main food. With the rapid increase of the global population and the decrease of arable land year by year, how to further increase rice production to meet the growing demand of mankind has become a major task in modern agricultural production. [0004] Tillering is an important agronomic trait affecting rice yield. The number of tillers formed by rice is the prerequisite for determining the number of spikes per mu, and the number of spikes is an important basis for yield. The cultivatio...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N15/82C12N5/10C12N1/15C12N1/19C12N1/21C12N15/11A01H1/00A01H5/00C12Q1/68
Inventor 朱立煌邹军煌翟文学潘学彪陈增祥张淑英江光怀
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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