Use of C60 polyhydroxy derivates in preventing and treating related disease of mitochondria damage

A mitochondrial, multi-purpose technology, applied in the fields of biology and pharmacy, can solve problems such as ineffectiveness

Inactive Publication Date: 2009-04-01
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For many years radiotherapy experts have been seeking ways to solve the side effects of radiation, using high oxygen and hypoxic radiotherapy, using radiosensitizers and protective agents, but the results are not great

Method used

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  • Use of C60 polyhydroxy derivates in preventing and treating related disease of mitochondria damage
  • Use of C60 polyhydroxy derivates in preventing and treating related disease of mitochondria damage
  • Use of C60 polyhydroxy derivates in preventing and treating related disease of mitochondria damage

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0064] The C 60 The preparation method of its polyhydroxy derivatives can adopt the methods known to those skilled in the art, such as the method of chemical synthesis,

[0065] Said compound can be used in the present invention in the form of pure substance, such as compound C 60 or Compound C 60 (OH) 24 ; In addition, mixtures of two or more of said compounds can also be used in the present invention, for example, C 60 and C 60 (OH) 24 A combination; or containing multiple C 60 A mixture of polyhydroxy derivatives, such as a mixture containing C 60 (OH) 24 、C 60 (OH) 23 、C 60 (OH) 22 Wait. In addition, considering the solubility of the compound, those compounds with a larger number of hydroxyl groups are preferred, such as C with n being 16-24 60 polyol derivatives.

[0066] The compounds of the present invention can also be used in the form of pharmaceutically or physiologically acceptable salts or esters. These salts or esters include, but are not limited to...

specific Embodiment

[0084] Material

[0085] Cell culture medium MEM, sodium pyruvate was purchased from Invitrogen; fetal bovine serum was purchased from Hyclone; penicillin, streptomycin, rotenone, antimycin A, NaN 3 , CoQ 1 , ATP, Triton X-100 and C 60 Purchased from Sigma Corporation; NaHCO 3 , EDTA, DMSO, absolute ethanol, and Tween-80 were purchased from China Pharmaceutical Group Shanghai Chemical Reagent Company; low-melting point agarose, Tris was purchased from Amresco; JC-1, DCF-DA was purchased from Molecular Probe; DCPIP was purchased from Merk Company; oxidative protein detection kit was purchased from Chemicon Company; protein lysate was purchased from Beyentian Institute of Biotechnology; anti-8-oxo-dG antibody was purchased from Trevigen Company, TRITC-linked IG anti-mouse antibody, Cy3-linked anti Rat IgG antibody was purchased from Jackson Company; GSH detection kit was purchased from Nanjing Jiancheng Biotechnology Research Institute.

[0086] Statistics and Data Analysis ...

Embodiment 1

[0088] Example 1C 60 (OH) 24 The effect of absorbing free radicals in vitro

[0089] In the present embodiment, adopt paramagnetic resonance spectrometer (EPR) to observe C 60 (OH) 24 In vitro absorption of free radical effects, the paramagnetic resonance spectrometer is produced by the German Brucker spectrometer company. The detected free radicals mainly include common superoxide free radicals, hydroxyl free radicals and lipid free radicals. Hypoxanthine and hypoxanthine oxidase generate superoxide radicals; hydroxyl radicals are generated by the Fenton reaction; lipid free radicals are generated by SDS-CuSO 4 system generated. Prepare different concentrations of C 60 (OH) 24 Aqueous solution, sequentially use ESR to detect the signal intensity of related free radicals. DMPO (dimethylpyrrole oxyone) and POBN (α-(1 oxy-4-pyridyl-N-tert-butyl nitrogen oxide)) were used as free radical scavengers, respectively.

[0090] figure 1 show C 60 (OH) 24 Effects of free rad...

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Abstract

The invention belongs to the biological and medical field and discloses an application of a C60 or C60 polyhydroxy derivative in preparing a combination for preventing and remedying the related diseases of mitochondrial injury (such as neurodegenerative disease or radiation sickness). The invention constructs a cellular model and an animal model with the related diseases of the mitochondrial injury, demonstrates the excellent efficacy of the C60 or C60 polyhydroxy derivative for preventing and remedying the related diseases of the mitochondrial injury and provides an effective approach for developing multifunctional drugs with mitochondria as a target point accordingly.

Description

technical field [0001] The present invention belongs to the field of biology and pharmacy, more specifically, the present invention relates to C 60 The use of the polyhydroxy derivatives in the prevention and treatment of diseases related to mitochondrial damage. Background technique [0002] Mitochondria are special organelles that widely exist in various eukaryotic cells, have circular DNA, and can replicate independently. It is not only the main place for the body to consume oxygen and produce ATP, but also has a variety of other extremely important physiological functions, including the production of reactive oxygen species such as superoxide anion, the regulation of cellular redox potential and signal transduction, the regulation of cell apoptosis and gene expression, etc. . As the regulatory center of cell metabolic network and signal transduction network, mitochondria have attracted increasing attention due to their important roles in growth, development, metabolism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/44A61K31/19A61P1/16A61P3/10A61P17/16A61P25/00A61P25/16A61P37/02A61P39/06
Inventor 刘健康蔡小青李文新
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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