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Method for inducing worm flying with fungal spore

A technology for carrying bacteria and attracting insects, which is applied in the direction of botany equipment and methods, fungi, devices for catching or killing insects, etc. It can solve the problems of unseen and other problems, and achieve the goal of reducing the degree of harm, reducing losses, and high spore production Effect

Active Publication Date: 2011-07-20
GUIZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no information has been found on the research on the use of entomogenic fungi to control storage pests

Method used

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  • Method for inducing worm flying with fungal spore
  • Method for inducing worm flying with fungal spore
  • Method for inducing worm flying with fungal spore

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0023] The test results showed that on the 7th day after the treatment, from the blank control carton water basin, the diseased corpses of tobacco beetles and tobacco powder moths covered with white hyphae were observed floating. First, microscopic examination of the sticky slices of the worms confirmed that it was fatally infected by Beauveria bassiana; second, it showed that the worms infected with the bacteria could migrate; third, it showed that the strains used were able to withstand high temperature and low humidity environments. However, in the B and C treatment boxes, there were significantly more insect corpses caused by infection. Along with the prolongation of test time, until 40 days in the control box, the sick insect corpses continue to increase, showing the long-term effect of its fungal infection.

[0024] Experimental example 2: Bacteria migration experiment 2 outside the warehouse

[0025] Place four cartons in the laboratory of the tobacco factory technical...

experiment example 2

[0026] The test results showed that the carton D, which was close to the source of insects, had significantly more carcasses of insects than the cartons B and C on days 7, 14, 21, and 30. Moreover, after spraying the room with the fungal spore powder test, no more rampant insects were seen.

[0027] Experimental example 3: Bacteria migration experiment in warehouse

[0028] Select three storage units of the same specification on the third floor of the alcoholization warehouse of the tobacco factory. The total amount of spore powder used per unit is 5g. See the location of the test carton Figure 1-3 , the black in the figure indicates that the spore powder was sprayed in the carton. Paste a piece of tobacco worm sex pheromone lure on the inner wall of each box, and then put a basin of clear water in the box. In this experiment, the effect of applying different spore powder concentrations and the information on the migratory distance of insects can be obtained.

experiment example 3

[0029] For the location of the test cartons in Warehouse 1, see figure 1 , in the figure: EA, EB, EC, ED distance is 20cm; spore powder dosage is 5.00g

[0030] For the location of the test cartons in Warehouse 2, see figure 2 , in the figure: the distance between CA, CE, and CD is 20cm; while the distance between CB is 40cm, and the amount of spore powder is 5.00g.

[0031] For the location of the test cartons in Warehouse 2, see figure 2 , in the figure: EA, EB, EC, ED are all 20cm apart; the amount of spore powder per box is 1.25g.

[0032] The test results showed that in the first week after the test, it was found that there were infected dead insects in the water basin of the blank control box 20cm away from the E treatment box in warehouse 1; Carcasses of tobacco beetle and tobacco meal borer covered with white mycelium. Pests infected with fungi can migrate a distance of 40 cm, which is the longest diagonal distance in the warehouse, that is, the distance from C ...

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Abstract

The present invention discloses a method for inducing the migratory flight of pests with bacterium, which comprises the following steps of: (1) selecting strains: (Beauveria bassiana) GZUIFR-GL5-08i and CCTCC No.M208202; (2) preparing spore powder; (3) setting a storage pest inducing box: a container for the reserve material of alcoholized tobacco leaves or food supplies and seeds are arranged inside and outside the storage; and the Beauveria bassiana spore powder is evenly spread in the container and simultaneously a pest external pheromone inducing core is hung in the container and a basin filled with fresh water is arranged in the container; and (4) detecting the situation of the migratory flight of pests with bacterium. The method plays the role in continuous control on the pests and can greatly reduce the damage degree thereof, thereby reducing loss.

Description

technical field [0001] The invention belongs to the field of biological control of pests, and in particular relates to a method for controlling pests in warehouses by adopting entomogenous fungal preparations. Background technique [0002] Tobacco beetle (Lasioderma Serricorne Fabricius) and tobacco meal borer (Ephesia elutella) are pests of tobacco storage worldwide, and they harm many crops. According to the survey, Tobacco A causes a loss of 1.64% of tobacco stored in my country every year. In the past, the prevention and control of tobacco pests mainly included physical methods of temperature control and mechanical insecticide, chemical methods of pesticide spraying and fumigation, monitoring methods of warehouse management pests, and microbial insecticide methods. In recent years, with the continuous improvement of people's awareness of ecological environment protection, the accumulation of knowledge and experience in biological control, and the prohibition of a large ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01M1/20A01M1/02A01N63/00C12N1/14
Inventor 刘爱英邹晓罗力胡海燕梁宗琦张晓敏杨辉吴让新王玉平施鸣
Owner GUIZHOU UNIV