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Uses of recombinant protein in preparing oral medication for preventing diabetes

A diabetes drug and recombinant protein technology, applied in the field of recombinant protein

Inactive Publication Date: 2009-05-13
XIAMEN UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The results of the study found that the binding of ProTα to Keap1 in the Nrf2-Keap1 complex is specific, and the mutant ProTα (MProTα) cannot perform this function

Method used

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  • Uses of recombinant protein in preparing oral medication for preventing diabetes
  • Uses of recombinant protein in preparing oral medication for preventing diabetes
  • Uses of recombinant protein in preparing oral medication for preventing diabetes

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0077] Construction and protein expression of recombinant ProTα: The PP restriction site in the fusion protein in pGEX-6p-1-ProTα was changed to the EK restriction site, so the upstream and downstream primers of ProTα were synthesized as follows:

[0078] Upstream primer: 5-CCCCTGGGATCCGATGACGATGACAAGTCAGACGCAGCCGTAGACA-3;

[0079] Downstream primer: 3pGEX Sequencing Primer (24bp): 5-CCGGGAGCTGCATGTGTCAGAGG-3

[0080] Expression build:

[0081] 1) For the PCR reaction of the target fragment, see the detection map of the ProTα PCR product figure 1 .

[0082] For enzyme digestion recovery of vector and target fragments, ligation, transformation and enzyme digestion identification of transformants, see figure 2 and image 3 .

[0083] Fusion protein expression, fusion protein purification method refer to molecular cloning manual and patent (200710009083.0)

[0084] expression results see Figure 4 .

[0085] Fusion protein permeation and Glutathione Sepharose 4B column p...

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Abstract

An application of a recombinant protein in the preparation of oral drugs for preventing diabetes relates to the recombinant protein. The invention provides the application of the recombinant protein in the preparation of the oral drugs for preventing the diabetes. The recombinant protein is the recombinant human prothymosin-alpha, and the preparation comprises the construction of the recombinant ProT-alpha, the protein expression and the expression construction. The recombinant protein has important regulatory function on the aspect of blood lipid and blood glucose metabolism through the oral delivery, thereby being capable of being used for preparing the oral drugs for preventing the diabetes.

Description

technical field [0001] The invention relates to a recombinant protein (human prothymosin alpha pro), in particular to a preparation method of the recombinant protein and its application in the preparation of oral medicine for preventing diabetes. Background technique [0002] With the improvement of people's living standards, long-term intake of high-sugar, high-fat and high-cholesterol foods will cause obesity, and obesity and overweight have become one of the worldwide epidemics. People have realized that eating too much processed food Energy surplus caused by carbohydrates is an important cause of obesity, because excessive carbohydrate intake will be converted into fat and deposited in the body. The effect of tolerance is reduced, and long-term accumulation will greatly increase the risk of suffering from type II diabetes. Animal experiments found that mice and rabbits fed high-sugar, high-fat and high-cholesterol food for a long time had their fasting blood sugar and b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P3/10
Inventor 周克夫王世媛韩伟吴汉州刘升发
Owner XIAMEN UNIV
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