Plastic embedding flaking method

Abstract

The invention discloses a myeloid tissue plastic embedding and pelletizing method which comprises the processes of fixing, deliming, dehydrating, soaking, embedding, slicing and dyeing, wherein, the processes of fixing, deliming, dehydrating and soaking are carried out under ultrasonic high frequency resonance. Compared with the traditional plastic embedding and pelletizing method, the plastic embedding and pelletizing method shortens the time from 50 hours to 15 hours approximately, saves about 3 working days and reduces the time for plastic embedding and pelletizing greatly, thereby reducing the economic burden of the patient and gaining precious time for remedying.

Description

technical field

[0001] The invention relates to a method for embedding a biological sample, in particular to a method for embedding a plastic sheet. Background technique

[0002] Plastic embedding means that its embedding agent is a mixed reagent of hydroxyethyl methacrylate, polyethylene glycol-400, and N-N=methylaniline, which is used to replace paraffin. Compared with paraffin sections, sections made of plastic embedding agents have the advantages of less cell shrinkage, clear structure, and high resolution. The traditional plastic-embedded sheet-making method adopts the following process steps:

[0003] 1. Fixation: fix tissue specimens with mercuric chloride, potassium dichromate and formaldehyde fixative (PCF). Fixed time>12h.

[0004] 2. Decalcification: decalcify the tissue specimen with 2% nitric acid by volume fraction for 2.5 hours.

[0005] 3. Dehydration: Gradient dehydration, tissue specimen dehydration, dehydration with volume fraction 80% ethanol 40mim...