Method for purifying hypericin

A technology of hypericin and process, which is applied in the fields of quinone separation/purification, organic chemistry, pharmaceutical formulation, etc., and can solve problems such as failure to effectively separate

Active Publication Date: 2009-06-17
BEIJING WBL PEKING UNIV BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods fail to effectively separate the two components

Method used

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  • Method for purifying hypericin
  • Method for purifying hypericin
  • Method for purifying hypericin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A. Extraction and enrichment:

[0058] Take 500g of the above-ground parts of Hypericum perforatum with flowers, grind it, and extract 3 times under reflux with 75% ethanol at 80°C. Press to recover the solvent, add water and ethanol to dilute to 4 times the weight of the medicinal material, and adjust to 50% alcohol content, mix well, and centrifuge to obtain a supernatant; soak the macroporous adsorbent resin with 95% ethanol for 24 hours and then pack it into a column to Wash the column with 95% ethanol until the effluent is no longer turbid after adding water; wash with distilled water until there is no alcohol smell; the supernatant is passed through the D-101 macroporous adsorption resin that has been pretreated above, and the amount of resin is 1% of the volume of the supernatant. / 5, stop loading the sample after the resin is saturated; elute with 2 times the volume of the resin, and discard the washing liquid; then elute with 40% ethanol, and discard the eluent...

Embodiment 2

[0068] A. Extraction and enrichment:

[0069] Take 1000g of the crude extract of Hypericum perforatum, add acetone to percolate and extract until it is colorless, recover the solvent under reduced pressure at 55°C, add ethanol to dissolve, filter off the precipitate, dilute the supernatant with water and ethanol to 8 times the weight of the medicinal material, and adjust to The alcohol content is 15%, mix well, and centrifuge to obtain the supernatant; soak the macroporous adsorption resin in 95% ethanol for 24 hours, then pack the column, wash the column with 95% ethanol until the effluent is no longer turbid after adding water. Wash with distilled water until there is no alcohol smell; the supernatant is passed through the pretreated HZ-816 macroporous adsorption resin, the amount of resin is 1 / 4 of the volume of the supernatant, and the sample is stopped after the resin is saturated; use 1 times the resin volume of water for elution, discarding the washing solution; followe...

Embodiment 3

[0079] A. Extraction and enrichment:

[0080] Take 200g of Hypericum perforatum, add methanol and ultrasonically extract until it is colorless, recover the solvent under reduced pressure at 40°C, add ethanol to dissolve, filter off the precipitate, dilute the supernatant with water and ethanol to 5 times the weight of the medicinal material, and adjust to the alcohol content Mix and centrifuge to obtain a supernatant; soak the macroporous resin in 95% ethanol for 24 hours, then pack the column, and wash the column with 95% ethanol until the effluent is no longer turbid after adding water. Wash with distilled water until there is no alcohol smell; the supernatant is passed through the pretreated LSI-106 macroporous adsorption resin, the resin volume is 1 / 5 of the volume of the supernatant, and the sample is stopped after the resin is saturated; use 1.5 times the resin volume of water for elution, discarding the washing solution; followed by elution with 30% ethanol, discarding ...

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Abstract

The invention discloses technology for purifying hypericin. The technology is to take hypericum perforatum, overground parts provided with flowers of other plants containing the hypericin, and a crude extract or flower of the plants as raw materials, and to prepare hypericin purified products of which the content is higher than 98 percent after solvent extraction, separation and purification. The purified products can be used as chemical standard substances, reference substances or raw materials of preparation.

Description

technical field [0001] The present invention relates to a purification process of hypericin, in particular to a kind of hypericin purified from Hypericum perforatum and other plants containing hypericin or from the crude extracts of the above-mentioned plants Purification process of freeze-dried powder. Background technique [0002] Hypericin is one of the antidepressant active ingredients in Hypericum perforatum L. and other plants containing hypericin. [0003] Hypericin is a benzodianthrone component, that is, 4,4', 5,5', 7,7'-hexahydroxy-2,2'-dimethyl-medium benzodianthrone. Molecular formula is C 30 h 16 o 8 , the molecular weight is 504.43. Blue-black needle-like crystal, easily soluble in pyridine and other organic amines, orange-red with red fluorescence, soluble in alkaline aqueous solution, almost insoluble in other organic solvents. It is a red solution when the pH is lower than 11.5, and it is a green solution with red fluorescence when the pH is higher tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/36C07C46/10A61K36/634
Inventor 段震文郭树仁何大林刘曦
Owner BEIJING WBL PEKING UNIV BIOTECH
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