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Method for recognizing erythrocyte chips

A red blood cell and debris technology, applied in the field of blood cell analyzers, can solve the problems of excessive red blood cell fragments and the influence of white blood cell counting results, and achieve the effect of improving accuracy

Active Publication Date: 2009-06-24
深圳迈瑞动物医疗科技股份有限公司
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Problems solved by technology

For other animals, there will also be a certain proportion of samples. Due to the action of hemolytic agents, the red blood cell fragments are too large, which will affect the white blood cell count results.

Method used

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  • Method for recognizing erythrocyte chips
  • Method for recognizing erythrocyte chips
  • Method for recognizing erythrocyte chips

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Embodiment Construction

[0049] Below according to accompanying drawing and embodiment the present invention will be described in further detail:

[0050] Since both the lymphoid peak and the red blood cell fragment peak are in the leftmost area of ​​the white blood cell histogram and their positions overlap, it is impossible to directly judge and filter out the interference of red blood cell fragments through the histogram position. Therefore, the present invention adopts the method of full width at half maximum to identify red blood cell fragments, that is, to identify lymph peaks and red blood cell fragments through pulse half maximum width distribution. The definition of particle pulse width at half maximum is as Figure 4 As shown, the particles pass through figure 1 The shown hematology analyzer micropore generates a pulse signal with a height of h, and the width of the pulse at half the height (1 / 2h) of the pulse signal is called the pulse width at half maximum.

[0051] The basic method of the...

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Abstract

A method for indentifying red cell fragments is used for blood cell analysis with cell counting by the resistance method, and comprises the following steps: A. counting white blood cells in a blood sample, drawing a white blood cell column diagram, and recording the full width at half maximum of each particle pulse during the process of particle pulse signal identification; B. locating an area A with possible existence of red cell fragments and an area B without any red cell fragments on the white blood cell column diagram, and determining whether the existence of red cell fragments interfering with white blood cell counting is true in the area A according to the comparison between the distribution of the full width at half maximum of particle pulse in the area A and in the area B; and C. in the case of disturbance caused by red cell fragments, determining the position, in which the interference peak of red cells ends, and correcting the count value of white blood cells according to the ending position of the interference peak of the red cells. The method achieves the effect of improving the accuracy of the result of white blood cell counting and the accuracy of white blood cell classification.

Description

technical field [0001] The invention relates to a blood cell analyzer using resistance method counting in the technical field of medical equipment, in particular to a method for identifying red blood cell fragments in the leukocyte testing process of human blood samples and other animal blood samples. Background technique [0002] As an instrument for counting and classifying blood cell parameters, blood cell analyzers have been widely used in clinical and laboratory fields, mainly providing white blood cell (WBC), lymphocyte percentage (Lymph%), monocyte percentage (Mon%), granulocyte Cell percentage (Gran%), red blood cell (RBC), platelet (PLT), mean corpuscular volume (MCV), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hemoglobin (HGB) And other test parameters, the test results are used for the doctor's diagnosis of the disease, so the test results are required to be accurate and reliable. [0003] in application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N33/48
Inventor 孔繁钢
Owner 深圳迈瑞动物医疗科技股份有限公司
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