Enzymolysis preparation of Cordyce militaris polypeptide and product

Abstract

The invention provides a method for preparing polypeptide of Cordyceps militaris by enzymolysis and product development. Cultivated Cordyceps militaris is dried at a temperature of between 37 and 72 DEG C and is crushed into powder, a biocatalyst (enzyme) of which weight is 0.007 to 0.05 percent of the weight of the Cordyceps militaris is added, and a zymoexciter is added at the same time. The hydrolysis is performed for 3 to 6h under water bath condition that the temperature is between 25 and 55 DEG C and the pH value is between 1.0 and 10.0, then the centrifugation and ultrafiltration are performed to obtain a clear yellowy Cordyceps militaris hydrolysate, and the hydrolysis rate is between 70 and 80 percent. The Cordyceps militaris hydrolysate contains a large amount of small molecular polypeptide, growth factors, esters (phospholipid, steroid compounds, fatty acid, and so on), more than 20 amino acids, trace elements and various effective medicinal components such as cordycepin, cordycepic acid, and so on. An obtained product can be applied to a plurality of aspects such as pharmacy, health care products and food, cosmetics, and so on, has convenient application, time saving and no pollution, and can develop a plurality of new products.

Description

Technical field: [0001] The invention relates to an extraction method of effective medicinal components of Cordyceps militaris, in particular provides an enzymatic hydrolysis preparation method and product development of a Cordyceps militaris polypeptide, belonging to the technical field of extracting effective components of traditional Chinese medicines. Background technique: [0002] Cordyceps militaris is rich in protein (30%), 21 kinds of trace elements, 9 kinds of vitamins and cordycepin, cordycepic acid, cordyceps polysaccharide, SOD enzyme and nucleic acid derivatives, etc. It has good medical value and health care function. The preparation of Cordyceps militaris products is still in the rough processing stage. The extraction of active ingredients is mostly by cooking, reflux, and Soxhlet extraction. It is limited to the extraction of cordycepin and Cordyceps polysaccharides, which takes a long time and consumes a lot. The high temperature will cause great damage to s...

AI Technical Summary

Problems solved by technology

The preparation of Cordyceps militaris products is still in the rough processing stage. The extraction of active ingredients is mostly by cooking, reflux, and Soxhlet extraction. It is limited to the extraction of cordycepin and Cordyceps polysaccharides, which takes a long time and consumes a lot. High temperature will cause great damage to some active ingredients, especially active polypeptide substances cannot be extracted, so only laboratory research can be carried out, and there is no high-tech deep-processing product that can be mass-produced