The invention relates to a biological enzyme desizing enzyme wash-bath process of pure cotton and cotton-containing type blended denim clothing. The process comprises the following steps: (1) performing desizing enzyme wash-bath: adding the denim clothing into an industrial washing machine, adding water, heating the water to 40-50 DEG C, adding desizing enzyme wash-bath enzyme into the industrial washing machine, controlling the pH value of the solution to be 6.0-8.0, opening the industrial washing machine, preserving the heat and rotating for 30-90 minutes, discharging the solution and washing for two times with clear water; (2) neutralizing with a reducing agent: adding the clothing on which monkey is sprayed into the industrial washing machine, adding water, heating the water to 25-40 DEG C, adding the reducing agent into the industrial washing machine, controlling the pH value of the solution to be 5.0-7.0, controlling the total hardness of water of the solution to be 70-150 ppm, opening the industrial washing machine, preserving the heating and rotating for 5-20 minutes, discharging the solution and cleaning for 1-2 times with clear water. Compared with the prior art, the process has the advantages that the process is time-saving, simple, easy to control and rational, no toxic or harmful substance is produced, the production efficiency is obviously improved, the product quality is more stable, and the production comprehensive cost is greatly reduced.