Method for extracting superoxide dismutase from cattle and sheep blood largly industrially and blade type agitator applied to same
A superoxide, large-scale technology, applied in mixer accessories, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of long temperature gradient processing time, large equipment investment, long heating time, etc., and achieve uniform temperature. And fast, reduce process cost, and is conducive to the effect of removal
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[0091] Example 1: Method for large-scale industrial extraction of superoxide dismutase from bovine blood
[0092] 1. Add 30g of sodium citrate anticoagulant to 10Kg of fresh bovine blood. Let stand at a low temperature of 4°C for 30 minutes, and after the solution is separated into layers, most of the supernatant is removed.
[0093] 2. Add an equal volume of distilled water, stir, hypotonic hemolysis for 30 minutes, and leave it for 40 minutes. After the solution is layered, the supernatant is subjected to ultrafiltration (using a polyacrylonitrile membrane with a pore size of 1 μm) to obtain 2000 ml of supernatant. liquid and 6500ml precipitation;
[0094] The precipitate was heated and stirred with a mixer with a heat source, so that it was rapidly heated to 40 ° C within 8 minutes, and after 20 minutes of heat preservation, it was stirred with a mixer with a cooling source while cooling, and it was quickly reduced to within 6 minutes. After centrifugation at room tempera...
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[0099] Example 2: Large-scale industrial extraction of superoxide dismutase from bovine blood
[0100] 1. Add 50g of sodium citrate anticoagulant to 10Kg of fresh bovine blood. Let stand at a low temperature of 4°C for 50 minutes, and after the solution is separated into layers, most of the supernatant is removed.
[0101] 2. Add an equal volume of distilled water, stir, hypotonic hemolysis for 30 minutes, and leave it for 50 minutes. After the solution is layered, the supernatant is subjected to ultrafiltration (using a polyacrylonitrile membrane with a pore size of 5 μm) to obtain 2500 ml of supernatant. liquid and 6000ml precipitation;
[0102] Heat the precipitate while stirring with a mixer with a heat source, so that it is rapidly heated to 45°C within 8 minutes, and after 10 minutes of heat preservation, it is cooled with a mixer with a cooling source while stirring, so that it is rapidly reduced to within 8 minutes. After centrifuging at room temperature at 5000 rpm ...
Example Embodiment
[0107] Example 3: Process for large-scale industrial extraction of superoxide dismutase from bovine blood
[0108] 1. Add 60g of sodium citrate anticoagulant to 10Kg of fresh bovine blood. Let stand at a low temperature of 4°C for 60 minutes, and after the solution is separated into layers, most of the supernatant is removed.
[0109] 2. Add an equal volume of distilled water, stir, hypotonic hemolysis for 30 minutes, and leave it for 60 minutes. After the solution is layered, the supernatant is subjected to ultrafiltration (using a polyacrylonitrile membrane with a pore size of 5 μm) to obtain 3500 ml of supernatant. liquid and 5800ml precipitation;
[0110] Heat the precipitate while stirring with a mixer with a heat source, so that it is rapidly heated to 55°C within 10 minutes, and after 30 minutes of heat preservation, it is cooled with a mixer with a cooling source while stirring, so that it rapidly drops to 55°C within 10 minutes. After centrifuging at room temperatur...
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