Method for extracting superoxide dismutase from cattle and sheep blood largly industrially and blade type agitator applied to same

Abstract

The invention discloses a method for extracting superoxide dismutase from cattle and sheep blood largly industrially, comprising the following steps of: carrying out the production steps of anticoagulation, hypotonic hemolysis, ultrafiltration, fast temperature rise and reduction, sedimentation, three-stage temperature treatment, salting-out, ultrafiltration, concentration, freeze drying, and the like on fresh cattle or sheep blood so as to simultaneously obtain two groups of superoxide dismutase finished products which are slightly different in specific activity and purity, wherein the fast temperature rise and reduction is realized through an agitator with a heat / cold source; the first-stage temperature treatment is carried out at 45-55 DEG C, the second-stage temperature treatment is carried out at 55-60 DEG C, and the third-stage temperature treatment is carried out at 60-65 DEG C. Compared with the prior art, the invention has the advantages of simple process, short production period, high yield coefficient and specific activity of the products, stable quality, low cost, excellent economic benefit, and the like.

Description

technical field [0001] The invention relates to a method for extracting superoxide dismutase, in particular to a large-scale industrialized method for extracting superoxide dismutase from blood of cattle and sheep. Background technique [0002] Superoxide dismutase (SOD) is a metalloenzyme that can catalyze the dismutation reaction of superoxide anion radicals. It is an extremely effective antioxidant that can resist the damage of superoxide anion radicals to cells. Studies have shown that: SOD may be one of the enzymes in the human body. SOD can be divided into two families from the structure, Cu-Zn-SOD is the first group, Mn-SOD and Fe-SOD are the second group, and Cu- Zn-SOD is the most well-known SOD, and it is a breakthrough for people to study the structure of SOD. With the in-depth expansion of SOD in clinical medicine, food and cosmetics, the demand for SOD is increasing. The preparation of bovine blood SOD will move from the laboratory to large-scale industrial pro...

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Problems solved by technology

[0008] A. Cu-Zn-SOD is easily denatured in the presence of organic solvents such as ethanol and chloroform, which will promote the conformational change of the egg self-quality

[0009] B. Organic solvents will cause about 8% loss of copper ions, and if the metal prosthetic group is removed, its stability will be greatly reduced, affecting the enzyme activity of SOD

[0010] C. This method consumes a large amount of organic reagents, increases production costs, and pollutes the environment

[0011] D. Expensive column chromatography system, column packing and high-speed centrifuge. The equipment investment is large, and after column chromatography, the yield of the product is greatly reduced, the ratio of input to output is extremely uneconomical, and large-scale chemical production cannot be realized

[0012] Even in the large-scale industrial production that does not use organic solvents to extract SOD, due to the heating in a large container, the difficulty of the direct thermal denaturation method is "uneven heating", and the temperature gradient treatment time for the supernatant is too long For example, there are some 6-level temperature gradient treatments with a total time of nearly 200 hours. Due to the long heating time, this kind of treatment not only easily leads to the denaturation of the target protein to be extracted, but also cannot "rapidly" heat up and down. Effective removal of foreign proteins, resulting in a decrease in the yield and specific activity of the target protein

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