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Preparation method of bionic nanofiber scaffold with electrical stimulation effects

A technology of bionic nanometer and fiber scaffold, which is applied in the fields of biomedical materials and nerve tissue engineering, can solve the problems of poor cell affinity of polylactic acid materials, aseptic inflammatory reactions, acidic degradation products, etc. The effect of good compatibility and good application prospects

Inactive Publication Date: 2016-06-15
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is one of the most widely used synthetic materials at present, but its application is limited due to the poor cell affinity of polylactic acid materials and the acidic degradation products, which can easily cause aseptic inflammatory reactions and are not conducive to cell growth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] Preparation of β-tricalcium phosphate

[0032] With a Ca / P molar ratio of 1:1.5, (NH 4 ) 2 HPO 3 Added dropwise to Ca(NO 3 ) 2 In the aqueous solution, after fully stirring, add polystyrene (dispersant) to the solution to make a mixed solution, and place it in a water bath, keep it at 35-38°C, and under strong stirring, add a pH regulator during the above stirring process (Ammonia water), adjust the pH to 7.0, let it stand overnight to obtain a suspension, filter under reduced pressure, wash with ethanol to obtain a filter cake, put it in a drying oven at 120° C. for 12 hours, and obtain β-TCP powder. Put the powder in the muffle furnace. Calcined at 800°C for 2 hours, cooled with the furnace, and taken out from the furnace to obtain β-TCP powder. Grinding to control the particle size of the β-TCP powder to be 10nm-100nm.

[0033] The molecular weight of polylactic acid used in the coaxial electrospun fiber scaffold (PLA / CS / β-TCP) prepared in the following exampl...

Embodiment 1

[0035] (1) Place tricalcium phosphate (β-TCP) in an alumina ring with a diameter of 2cm and a height of 0.5cm, at 2KV / cm -1 Polarization was carried out in air at 100° C. for 1 hour under the action of a direct current electric field, and the obtained polarized β-TCP was used for future use.

[0036] (2) At room temperature, a composite solvent with a volume ratio of dichloromethane (DCM) and N-N-dimethylformamide (DMF) of 9:1 is used as a spinning solvent, and polylactic acid (PLA) is added to dissolve it to make a mass concentration of 5% polylactic acid, then add 2% mass concentration of electropolarized β-TCP sodium citrate dispersion, as the cortex pre-electrospinning solution 1; mass concentration is 2% chitosan acetic acid aqueous solution, add bFGF0.5 μg, After magnetic stirring for 6 hours, the drug was uniformly dispersed and used as the core layer pre-electrospinning solution 2.

[0037] (3) Put the two pre-electrospinning solutions prepared above into syringes wit...

Embodiment 2

[0040] (1) Place tricalcium phosphate (β-TCP) in an alumina ring with a diameter of 4cm and a height of 1.5cm, at 4KV / cm -1 Polarization was carried out in air at 200° C. for 2 hours under the action of a DC electric field, and the obtained polarized β-TCP was used for future use.

[0041] (2) At room temperature, a composite solvent with a volume ratio of dichloromethane (DCM) and N-N-dimethylformamide (DMF) of 2:1 is used as a spinning solvent, and polylactic acid (PLA) is added to dissolve it to make a mass concentration of 20% polylactic acid, then add the electropolarized β-TCP sodium citrate dispersion with a mass concentration of 5%, as the cortex pre-electrospinning solution 1; add bFGF0.75 μg to the chitosan acetic acid aqueous solution with a mass concentration of 5%, After magnetic stirring for 6 hours, the drug was uniformly dispersed and used as the core layer pre-electrospinning solution 2.

[0042] (3) Put the two pre-electrospinning solutions prepared above in...

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Abstract

The invention discloses a preparation method of a composite bionic nanofiber scaffold with electrical stimulation effects. Beta-tricalcium phosphate is polarized for 0.5-3 hours in air with the effects of a 2-8KV / cm-1 direct-current electric field at 200-600 DEG C, and polarized beta-TCP is obtained; polylactic acid is dissolved in an organic solvent, the polarized beta-TCP powder is added, and PLA / beta-TCP pre-electrospinning liquid 1 is prepared; a medicament with biological activity is added in an aqueous solution of chitosan-acetic acid with uniformly stirring for dispersion, and CS / medicament with biological activity pre-electrospinning liquid 2 is prepared; PLA / CS / beta-TCP is prepared by coaxial electrospinning. The nanofiber scaffold with electrical stimulation effects has a double layer internal structure and a large surface area, in order to realize timely and proper controlled release of neurotrophic factors and various medicaments; the nanofiber scaffold has the advantages of good biological compatibility, stable performance, preservation convenience, and good application prospects in peripheral nerve repair and other biomedical fields.

Description

technical field [0001] The invention belongs to the fields of biomedical materials and nerve tissue engineering, and relates to an electrospinning technology to prepare a bionic nanofiber support with electrical stimulation. Background technique [0002] Peripheral nerve injuries are very common in clinical practice, especially the repair of long-distance nerve defects has always been a difficult problem in the medical field. So far, several processes such as nerve anastomosis, autologous nerve transplantation, allogeneic nerve transplantation, autologous non-nerve tissue transplantation, and biomaterial replacement have been experienced. solution. The blended scaffold material prepared by electrospinning technology has high porosity and large specific surface area, which can hybridize and complement the structure of natural materials and synthetic materials, so the electrospun nanofiber material with good biocompatibility and degradability is used to inoculate seeds Cells...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/18A61L27/20A61L27/50A61L27/22A61L27/54D01D5/00D01D5/34D01D1/02
CPCA61L27/12A61L27/18A61L27/20A61L27/227A61L27/50A61L2300/252A61L2400/12A61L2430/32D01D1/02D01D5/0015D01D5/0061D01D5/0076D01D5/34C08L67/04C08L5/08C08L89/00
Inventor 徐海星李依萍许沛虎黄志军张凌溪李燕郭兴蕾吴峰政
Owner WUHAN UNIV OF TECH
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