Cosmetic or plastic composition comprising multipotent stem cells derived from human adipose tissue, fibroblast, and adipose or adiopocyte

A fibroblast and adipose tissue technology, applied in bone/connective tissue cells, animal cells, tissue culture, etc., can solve problems such as stem cell plastic surgery and plastic surgery with less effort

Inactive Publication Date: 2009-07-01
RNL BIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] While many efforts have been made so far to use adipose-derived stem cells for medical applications includ...

Method used

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  • Cosmetic or plastic composition comprising multipotent stem cells derived from human adipose tissue, fibroblast, and adipose or adiopocyte
  • Cosmetic or plastic composition comprising multipotent stem cells derived from human adipose tissue, fibroblast, and adipose or adiopocyte
  • Cosmetic or plastic composition comprising multipotent stem cells derived from human adipose tissue, fibroblast, and adipose or adiopocyte

Examples

Experimental program
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Embodiment 1

[0033] Example 1: Isolation of multiplastic adult stem cells from adipose tissue and preparation of compositions for plastic surgery

preparation Embodiment 1

[0035] Uniformly resuspend the adipocyte-containing suspension obtained by liposuction in saline with an appropriate amount of saline, and place a sufficient amount of the resuspended suspension in a cell culture flask or a shaker flask Incubate by standing or shaking. A minimum of 6-12 hours is required for static cultures. Next, the cell layer (MSCs from adipose, fibroblasts) attached to the surface of the culture flask was collected by treating with trypsin ( figure 1 ). At this time, the cells suspended in a small amount of saline are directly collected for use, or the cell layer collected from the saline is centrifuged at 1000 rpm for 10 minutes to use only the pelleted pellet in the case where the volume of the cell layer should be reduced . The separated cell layer contains adult stem cells and fibroblasts. The composition for cosmetic or plastic surgery containing adult stem cells, fibroblasts, fat and adipocytes according to the present invention is prepared by mi...

preparation Embodiment 2

[0037] Pluriplastic stem cells were isolated and purified from human adipose tissue. That is, the isolated human adipose tissue was washed 3 times with PBS and then finely dissected. The dissected tissue was digested at a ratio of 1:3 (adipose tissue:DMEM) in DMEM-LG (low glucose) medium supplemented with type 1 collagenase (1 mg / ml) at 37° C. for 1 hour. After washing the digested tissue with PBS, it was centrifuged at 1000 rpm for 5 minutes. The supernatant was aspirated, and the remaining pellets on the bottom were washed with a mixture of 10% FBS and DMEM-LG (low sugar) at a ratio of 1:1, followed by centrifugation at 1000 rpm for 5 minutes. The resulting pellet was filtered through a 100 μm mesh to remove debris and washed with PBS. The obtained cells were cultured in DMEM medium (10% FBS, 2 mM NAC, 0.2 mM ascorbic acid). After one night, unattached cells were washed with PBS and incubated in K-NAC medium (keratinocyte-SFM medium + 2 mM NAC + 0.2 mM ascorbic acid + 0...

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Abstract

The present invention relates to a composition for cosmetic or plastic surgery, containing adult stem cells derived from human adipose tissue, fibroblasts and adipose or adiopocytes, and a method for preparing the same. The composition for cosmetic or plastic surgery according to the present invention can be prepared by culturing an adipose-containing suspension obtained by liposuction and then collecting cell layers containing both adipose-derived adult stem cells and fibroblasts which are attached to the surface of the culture flask, followed by adding adipocytes to the collected cell layers. The composition according to the present invention is useful for cosmetic purposes such as skin tightness improvement, wrinkle and flabby skin improvement, and depressed area restoration through plastic surgery as well as useful for the formation of new breast tissue.

Description

technical field [0001] The present invention relates to a composition for cosmetic or plastic surgery and a preparation method thereof, the composition contains adult stem cells, fibroblasts and fat or adipocytes (Adiipocyte) from human adipose tissue. Background technique [0002] Stem cells refer to cells that have both self-replication ability and the ability to differentiate into at least two types of cells, which can be divided into totipotent stem cells, pluripotent stem cells and multipotent stem cells ). [0003] Recently, adipose tissue was found to be a new source of multiplastic stem cells (Cousin et al., BBRC., 301:1016, 2003; Miranville et al., Circulation, 110:349, 2004; Gronthos et al., J. Cell Physiol., 189: 54, 2001; Seo et al., BBRC., 328:258, 2005). That is, it was reported that human adipose tissue obtained by liposuction contained a group of undifferentiated cells with the ability to differentiate into adipogenic cells, osteoblasts, myogenic cells and ...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N5/077C12N5/0775
CPCC12N2501/01C12N5/0667C12N2500/32C12N2501/33C12N5/0653C12N2501/39A61K35/12C12N5/0656A61L27/60C12N5/0662
Inventor 罗正灿
Owner RNL BIO
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