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Preparation of Gracilaria gigas Harvey polysaccharides

A technology for Gracilaria and polysaccharide, which is applied in the field of preparation of Gracilaria polysaccharide, can solve the problems of few research reports on polysaccharide extraction and analysis, and achieves the effects of low preparation equipment and site requirements, high yield and low cost

Inactive Publication Date: 2011-03-16
HAINAN STAND BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gracilaria is one of the species of algae cultured in the coastal areas of Hainan, Guangdong, Zhejiang, and Taiwan. It is mainly used as feed for fish farming and raw material for food glue production. There are few research reports on the extraction and analysis of polysaccharides on it.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 2000g of Gracilaria algae powder and add 10L of deionized water for heating and extraction. The extraction temperature is 85°C and the extraction time is 4h. Add 10L deionized water to the filter residue, add 0.5mol / L hydrochloric acid to adjust the pH value to 4.8, and heat to a temperature of 50°C±2°C. Add 200,000 international units (specific activity) compound enzyme per kg of Gracilaria algae powder for enzymolysis, add 400,000 international units (specific activity) compound enzyme, the compound enzyme contains cellulose with a weight ratio of 2-1:1-2 Enzyme and hemicellulase; after stirring well, hydrolyze for 120min at pH 4.8 and temperature 50°C±2°C. After enzymolysis, heat to inactivate the enzyme, cool down, and filter to obtain filtrate ②9.0L.

[0031] Merge filtrate 1. and filtrate 2. obtain 17.5L filtrate altogether, adjust the pH value to 6.8 with 18% (weight percentage) sodium hydroxide solution, temperature is 50 ℃ ± 2 ℃, add 180,000 international...

Embodiment 2

[0037] Take 2000g of Gracilaria algae powder and add 20L of deionized water for heating and extraction. The extraction temperature is 95°C and the extraction time is 6h. Add 15L of deionized water to the filter residue, add 0.5mol / L hydrochloric acid to adjust the pH to 3.5, and heat to a temperature of 55°C±2°C. Add 150,000 international units (specific activity) compound enzyme per kg of Gracilaria algae powder for enzymolysis, add 300,000 international units (specific activity) compound enzyme, the compound enzyme contains cellulose with a weight ratio of 2-1:1-2 Enzyme and hemicellulase; after stirring well, hydrolyze for 150min at pH 3.5 and temperature 45°C±2°C. After enzymatic hydrolysis, heat to inactivate the enzyme, cool down, and filter to obtain filtrate ②13.0L.

[0038]Merge filtrate 1. and filtrate 2. obtain 30.0L filtrate altogether, adjust the pH value to 8.0 with 18% (weight percentage) sodium hydroxide solution, temperature is 45 ℃ ± 2 ℃, add 100,000 interna...

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PUM

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Abstract

The invention discloses a preparation method for a Gracilaria polysaccharide. The Gracilaria polysaccharide is separated and extracted from common Gracilaria, Gracilaria, Gracilariaceae, Gigartinales, Florideae, and Rhodophyta. The molecular weight is between 500 and 50,000 Daltons. The Gracilaria polysaccharide solid is from faint yellow to white. The product can be developed into new anti-tumorand anticancer medicines. The preparation method is reliable and feasible and is suitable for the large-scale production. The preparation method has no environmental pollution and has low cost.

Description

technical field [0001] The invention relates to a preparation method of Gracilaria polysaccharide. Background technique [0002] Gracilaria is a perennial large red algae, belonging to Rhodophyta, Euerythrophyta, Cedaraceae, Gracilaceae, Gracilaria genus, about 100 species of Gracilaria have been reported in the world, and there are more than 10 common species of Gracilaria in my country. kind. Gracilaria is one of the species of algae cultured along the coasts of Hainan, Guangdong, Zhejiang, and Taiwan. It is mainly used as feed for fish farming and raw material for food rubber production. There are few research reports on the extraction and analysis of polysaccharides. After continuous exploration of the extraction process of Gracilaria polysaccharides and combining some previous experiences, we invented Gracilaria polysaccharides. [0003] Gracilaria polysaccharides are one of the important biologically active components in Gracilaria. Polysaccharides are a class of impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P39/06A61P7/02A61P31/00C08B37/04A61P35/00
Inventor 伍曾利吴家强王海生
Owner HAINAN STAND BIO TECH