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Maize genes for controlling plant growth and organ size and their use in improving crop plants

A plant, corn technology, applied in the field of molecular biology, can solve the problem of undetected

Inactive Publication Date: 2009-07-15
PIONEER HI BRED INT INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Arabidopsis ARGOS is naturally expressed at low levels in a variety of young tissues including roots, flower stems, flowers, young rosette leaves, and siliques, but is not detected in mature leaves

Method used

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  • Maize genes for controlling plant growth and organ size and their use in improving crop plants
  • Maize genes for controlling plant growth and organ size and their use in improving crop plants
  • Maize genes for controlling plant growth and organ size and their use in improving crop plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0234] Example 1. Isolation of ARGOS sequences

[0235] Related ARGOS genes were found using conventional methods for identifying all members of a gene family. The protein sequences of all known members of the various groups of this gene family were prepared. This data includes sequences from other species. These species were searched in proprietary maize sequence datasets and non-redundant sets of overlapping results were identified. In a stepwise manner, the nucleotide sequence of any gene of interest is obtained, the database is searched and a non-redundant set of all overlapping results is retrieved. These sets of protein results are then compared to the nucleotide results. If the gene family is complete, all protein results are included in the nucleotide results. The ARGOS gene family consists of 3 Arabidopsis genes, 8 rice genes, 9 maize genes, 9 sorghum genes and 5 soybean genes. For a dendrogram illustration of the interrelationships of proteins encoded by the...

Embodiment 2

[0236] Example 2. ARGOS sequence analysis

[0237] The ZmARGOS polypeptide of the present invention has common characteristics with ARGOS genes of various plant species. The relationship of the genes in multiple plant species is expressed in the form of comparison, as shown in Figure 2. image 3 Contains ZmARGOS 1, 2, 3 and AtARGOS1 (SEQ ID NO: 2, 4, 6 and 26). The protein encoded by the ARGOS gene has a highly conserved proline-rich region near the C-terminus. The N-terminus is more variable. The protein is relatively short, containing an average of 110 amino acids.

Embodiment 3

[0238] Example 3. Transformation and regeneration of transgenic plants

[0239] Bombardment with a plasmid containing the ZmARGOS sequence operably linked to the drought-inducible promoter RAB17 promoter (Vilardell et al. (1990) Plant Mol Biol 14:423-432) and the selectable marker gene PAT (which confers resistance to the herbicide Bialaphos) Immature maize embryos from greenhouse donor plants. Alternatively the selectable marker gene can be provided on a separate plasmid. Transform according to the following steps. The medium recipe is shown below.

[0240] Preparation of target tissue:

[0241] Ears were dehulled and surface sterilized with 30% Cloros bleach plus 0.5% Micro detergent for 20 minutes and rinsed twice with sterile water. Immature embryos were minced and placed axis-side down (scutellum side up), 25 embryos per plate, placed in 560Y medium for 4 hours and then arrayed in a 2.5-cm target area for bombardment .

[0242] DNA preparation

[0...

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PUM

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Abstract

The present invention provides polynucleotides and related polypeptides of the ZmARGOS gene family. The invention provides genomic sequence for the ZmARGOS genes. ZmARGOS is responsible for controlling plant growth, organ size and yield in crop plants.

Description

field of invention [0001] The present invention generally relates to the field of molecular biology. Background of the invention [0002] The domestication of many plants has been associated with large increases in yield. The vast majority of phenotypic variation that occurs in natural populations is persistent and the result of multiple genetic influences. Identifying specific genes associated with large yield changes in domesticated plants has become a major direction of agricultural research. [0003] The auxin-regulated genes (ARGOS) associated with organ size genes in Arabidopsis (Arabidopsis), a family of genes associated with plant changes associated with increased crop yield, are inducible by auxin. This gene is responsible for regulating cell proliferation and organ growth. Arabidopsis ARGOS is naturally expressed at low levels in various young tissues including roots, flower stems, flowers, young rosette leaves and siliques, but is not detected in mature leaves....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/43C12N15/82
CPCC07K14/415C12Q1/6895C12N15/8261Y02A40/146C12N15/8273
Inventor M·郭W·布鲁斯R·古普塔C·R·西蒙斯
Owner PIONEER HI BRED INT INC